转SCK基因大米对小型猪生长发育的影响

The effect of rice genetically modified with cowpea trypsin inhibitor gene on the growth and development of minipigs

为观察和比较转SCK基因大米和亲本大米对机体生长发育的影响,从而评价转SCK基因大米的营养价值,选用15头雄性断乳中国实验用小型猪为试验对象,随机分成3组,分别是正常对照组(NG)、亲本大米对照组(CG)、转基因大米组(GG),饲料根据实验目的配制,共饲养62d。观察3组动物体格发育(体重、体长、体高、胸围)和脏器发育(脏器重、大体病理和组织病理)等方面的差异。结果表明,亲本大米对照组和转基因大米组动物的体格发育均优于正常对照组;3组动物脏器发育差异不明显,大体病理和组织病理均未观察到转SCK基因大米具有明显的非期望效应。说明外源性基因的插入未改变大米的营养价值或产生对动物的不良影响,转SCK基因大米和亲本大米在动物喂养方面基本满足“实质等同性”的要求。

In order to assess and compare the nutritional value of rice genetically modified with cowpea trypsin inhibitor gene (GM rice) and to see the effect of this rice on the growth and development of animals, 15 weaning male minipigs weighed 6 22±0 42kg were divided into three groups and 5 for each group,: (1) Normal group (NG), fed a commercial pig diet, (2) Control or parental rice group ( CG), fed a diet contain 70% parental rice, (3)GM rice group (GG), fed a diet contain 70% GM rice. All minipigs were housed individually in the pen, and had ad libitum access to feed and water. The feeding period was 62 days, the growth (weight, height, body length, breast circumference, ect.) and food intake were determined at regular intervals. All the animals were sacrificed at the end of the experiment and the parameters of growth and development were examined, and compared among the three groups. The results showed that there were no significant difference in the growth and development of body, organ weight and histopathologic examination of tissues in CG and GG group, the body weight gain of minipigs in CG and GG group was higher than that in NG group ( P< 0 05). The results suggest that the feeding value of GM rice and parental rice were similar in this study, and no detrimental unexpected effects were observed in minipigs fed rice genetically modified with cowpea trypsin inhibitor gene.