赭曲霉毒素A分析方法进展

Methods for ochratoxin A analysis

赭曲霉毒素A(OchratoxinA,OTA)是曲霉属和青霉属的某些菌种产生的一种具有致癌、致畸、免疫抑制和肝肾毒性的有毒代谢产物,世界上许多国家已制定了食品中OTA的相关法规和标准,而准确、可靠、灵敏的分析OTA方法是法规标准实施的重要依据。有机溶剂与酸(或碳酸氢钠)的混合溶液是提取食品中OTA的常用溶剂系统;含有OTA提取液的净化手段包括液液分配、固相柱萃取和免疫亲和层析等;反相液相色谱配荧光检测器、酶联免疫吸附法和液相色谱质谱联机是分析OTA的常用方法,本文就OTA的检测方法进行综述。

The mycotoxin ochratoxin A (OTA) is produced by some strains of fungi Aspergillus alutaceus and Penicillium verrucosum and has carcinogenic, nephrotoxic, teratogenic and immunosuppressive properties. The levels of OTA in foodstuffs are regulated in several countries, so reliable and sensitive methods are necessary for its determination. Procedures for extraction of OTA from foods generally use an organic solvent in the presence of acid or an extraction solvent containing aqueous sodium bicarbonate. Cleanup procedures include partition into aqueous sodium bicarbonate, solid phase extraction (SPE) columns and immunoaffinity chromatography. Widely used determinative procedures include reversed phase liquid chromatography (LC) with detection by fluorescence, enzyme linked immunosorbent assay (ELISA) and HPLC/tandem mass spectrometry. Methods for OTA analysis are reviewed.