卡介菌多糖核酸提高复发性生殖器疱疹患者CD8^+T细胞的细胞因子表达

Enhancement of BCG-PSN on the cytokines in CD8^+ T lymphocytes in the peripheral blood of patients with recurrent genital herpes

目的 研究卡介菌多糖核酸 (BCG PSN)对复发性生殖器疱疹 (RGH)患者外周血CD8+T细胞IL 12、IFN γ、IL 4表达的影响。方法 应用流式细胞仪对 4 5例RGH患者和 15名健康志愿者外周血CD8+ T细胞IL 12、IFN γ和IL 4的表达进行检测。结果 治疗前RGH患者外周血IFN γ和IL 12阳性CD8+ T细胞百分率均显著下降 (P <0 .0 5 ) ,Tc1 Tc2平衡失调 (P <0 .0 5 )。治疗后BCG PSN组外周血IL 12和IFN γ阳性CD8+ T细胞百分率显著升高 (P <0 .0 5 ) ,Tc1 Tc2恢复平衡。病例对照组IL 12、IFN γ及IL 4阳性CD8+ T细胞百分率于治疗前后变化差异均无显著性 (P >0 .0 5 )。BCG PSN组与病例对照组治疗前后IL 12、IFN γ阳性CD8+ T细胞百分率间差异均有显著性 (P <0 .0 5 )。BCG PSN组复发率较低 ,复发病情较轻。结论 RGH患者存在以Tc1水平低下为主的Tc1 Tc2比例失衡和IL 12表达水平低下 ;BCG PSN通过上调外周血CD8+ T细胞IFN γ、IL 12的表达 。

Objective To investigate the effects of polysaccharide nucleic acid of Bacillus Calmette Guerin(BCG-PSN) on the cytokines (IL-12, IFN-γ and IL-4) in CD8^+ T lymphocytes in patients with recurrent genital herpes(RGH). Methods Flow cytometric analysis was employed to study intracellular cytokines(IL-12, IFN-γ and IL-4) in CD8^+ T lymphocytes in the peripheral blood of 45 patients with RGH (among them 30 cases were put under BCG-PSN injection and 15 cases as case-control) and another 15 healthy volunteers as normal control. Results Before treatment, the percentage of IFN-γ-producing and IL-12-producing CD8^+ T lymphocytes were significantly lower (P<0.05), while the ratio of Tc1/Tc2 was lower in RGH patients than that in healthy volunteers (P<0.05). The percentage of IFN-γ-producing and IL-12-producing CD8^+ T lymphocytes were significantly increased in RGH patients with BCG-PSN group (P<0.05) and the ratio of Tc1/Tc2 in these patients recovered normal. The percentage of IFN-γ-producing, IL-12-producing and IL-4-producing CD8^+ T lymphocytes were not significantly changed in case-control group (P>0.05). The differences between the percentage of IFN-γ-producing and IL-12-producing CD8^+ T lymphocytes before and after treatment were significantly higher in the BCG-PSN group than that in the case-control group. There were a higher rate of clinical control and milder recurrence in the BCG-PSN group than that in the case-control group. Conclusion There are Tc1/Tc2 inbalance with a decline of IFN-γ-producing subsets and a low level of IL-12-producing CD8^+ T lymphocytes in RGH patients. Usage of BCG-PSN can upregulate the level of IFN-γ-producing and IL-12-producing CD8^+ T lymphocytes and adjust the Tc1/Tc2 inbalance in RGH patients. [