新的生化痕量分析手段——超声悬浮技术

Novel Trace Bioanalysis Technology——Ultrasonic Levitation

超声悬浮技术是利用声辐射力将物体悬浮在超声驻波场声压结点处的无容器处理技术 ,该技术能够以非接触的方式处理体积为几 μL甚至几十 p L的样品 ,避免因容器壁的不确定性吸附、记忆效应和污染而引起的分析物的损失 ,排除由于容器壁与样品间的相互作用对细胞反应的干扰以及容器壁引起的光学干扰 ,且对被悬浮物体的物理化学性质无特殊要求 ,是基于单颗粒或小液滴研究的强有力工具 ,特别适合于材料的深过冷 (远离凝固平衡状态 )研究和小体积痕量分析 ,可使检测极限降低 1～3个数量级。

Ultrasonic levitation technology, holding small objects at pressure node by acoustic radiation forces, is a containerless processing technology that excludes the unspecific absorption of container walls, avoids the intrusion into cell reaction through interactions between container wall and samples, shuns the optical interference caused by container walls, and has no special request on the physical property of levitated objects. It is a powerful tool for a variety of studies based on single particle or droplets with volume in μL down to pL, especially suitable for materials supercooling processing and small-scale trace analysis.Applications and developments of ultrasonic levitation in trace bioanalysis are introduced in this contribution.