葡萄球菌医院感染及耐药谱分析

Antibiotics Resistance of Staphylococcus in Nosocomial Infection

目的 了解我院 3年来导致医院感染的葡萄球菌分类及耐药性。方法 采用常规培养方法对我院医院感染患者的各类标本进行分离培养 ,用美国Micro WalkAway 4 0细菌鉴定仪对分离的细菌进行鉴定 ,然后用纸片扩散法进行药物敏感试验 ,并进行统计学分析。结果 共分离葡萄球菌 2 16株 ,其中以金黄色葡萄球菌占第 1位为2 4 5 % ,其他 16 3株为凝固酶阴性葡萄球菌 ;甲氧西林耐药金黄色葡萄球菌 (MRSA)检出率为 39 6 % ;耐甲氧西林凝固酶阴性葡萄球菌检出率为 36 2 % ;耐药率监测表明耐甲氧西林葡萄球菌 (MRS)株对 10种常用抗菌药物耐药率远远高于MSS株。结论 临床细菌室工作人员应高度重视MRS的检测和报告 ,为临床医师提供可靠的诊断和治疗依据 。

OBJECTIVE To investigate the distribution and drug resistance of Staphylococcus in nosocomial infection during the last three years in our hospital. METHODS Strains collected from daily specimens were identified with Micro WalkAway 40, and then the drug resistances were measured by standard K B methods. All data were analyzed statistically. RESULTS Among 216 Staphylococcus strains collected, Staphylococcus aureus were predominant [24 5% (53/216)], and the remains were all coagulase negative Staphylococcus. Meticillin resistant Staphylococcus aureus (MRSA) accounted for 39 6%(21/53), and meticillin resistant coagulase negative Staphylococcus(MRCNS) accounted for 36 2%. The drug sensitivity results showed that meticillin resistant Staphylococcus (MRS) was highly resistant to ten commonly used antibiotics than meticillin sensitive Staphylococcus (MSS). CONCLUSIONS For effective supervision and control of Staphylococcus infection, detection of MRS should be paid much attention by clinical microbiology laboratory.