胰腺癌和原发性肝癌组织中HBVDNA原位杂交的研究

Study on in-situ hybridization of HBVDNA in pancreatic carcinoma and primary hepatocarcinoma tissues

目的研究胰腺癌和肝癌组织HBVDNA状况及其病因学意义。方法51例胰腺癌和37例肝癌手术切除标本经10.0%福尔马林固定后常规制作石蜡包埋切片,HBVDNA染色方法为原位杂交法。结果16例胰腺癌(31.4%)HBVDNA阳性,阳性病例血清HBsAg均阳性;28例肝癌(75.6%)HBVDNA阳性,阳性病例AFP值阳性率及肝硬化程度明显地高于阴性病例,HBVDNA与胰腺癌和肝癌其他临床病理特征无明显关系。结论HBV感染除与肝癌发生密切相关外,也可能是胰腺癌发生的一个重要致癌因素。

Objective:To study the in-situ hybridization of HBVDNA and detect its etiological significances on the carcinogenesis of pancreatic and hepatic tissues.Methods:HBVDNA was detected by in-situ hybridization on the formalin-fixed and routinely paraffin-embedded sections of the surgical resection specimen with pancreatic car-cinoma(n=51)and hepatocarcinoma(n=37).Results:The positive rate of HBVDNA was31.4%(16/51)in pancre-atic carcinoma,the positive cases showed serum HBsAg(+).The positive rate of HBVDNA was75.6%(28/37)in hep-atocarcinoma,the positive cases showed significantly higher in the positive rate of AFP in serum and severity degree of liver cirrhosis than in negative ones.No significant relationships were found among HBVDNA status and other clinicopathological characteristics of pancreatic carcinoma and hepatocarcinoma.Conclusions:Besides close corre-lation between HBV infection and the carcinogenesis of liver tissues,HBV infection may also be an important etio-logical factor on the carcinogenesis of pancreatic tissues.