ELISA法和MEIA法测定HBV血清标志物的比较研究

Comparative study on ELISA and MEIA methods used in detecting serum HBV markers

目的比较酶联免疫吸附法(ELISA)和微粒子酶免疫分析法(MEIA)对HBV血清标志物的检测性能。方法用ELISA法和MEIA法对HBV的血清标志物(HBsAg,HBeAg,Anti-HBc和Anti-HBe)分别进行检测。结果对1140例病人的血清进行乙肝标志物的检测,MEIA法阳性率高于ELISA法。与MEIA法相比较,ELISA法检测HBsAg的相对灵敏度达97.5%,Anti-HBc(94.9%)和HBeAg(73.4%)次之,Anti-HBe最低,为41.7%。对一些低滴度的HBeAg/Anti-HBe的HBV感染者,ELISA法可以引起假阴性。结论对于血清乙肝标志物的检测,MEIA法和ELISA法均有较高的特异性,但MEIA法敏感性优于ELISA法。

Objective:To compare the positive rates of ELISA and MEIA methods used in detecting serum HBV markers.Methods:ELISA and MEIA methods were used to detect HBV markers including HBsAg,HBeAg,Anti-HBc and Anti-HBe.Result:The positive rate of MEIA was higher than that of ELISA on detecting HBV marker of1140patients sera.Comparing with MEIA,ELISA had different relative sensitivities on detecting different markers,which were97.5%(HBsAg),94.9%(Anti-HBc),73.4%(HBeAg)and41.7%(Anti-HBe)respectively.Regarding HBV patients with low titers of HBeAg/Anti-HBe,ELISA could result in false negativity.Conclusions:MEIA is more sensitive than ELISA on the detection of serum HBV markers.