运动训练对周围神经损伤大白鼠神经功能恢复的影响

Effect of exercise training on neural functional recovery of white rats after peripheral nerve injury

目的:研究在周围神经损伤的动物模型上,运动训练对神经功能恢复的影响。方法:实验在延边医学院附属医院中心实验室完成。用60只Wistar雄性大白鼠,分离出坐骨神经,在胫神经和腓神经分支前的部位用止血钳压迫制造坐骨神经损伤模型,实验动物分为3组,利用动物用电动跑步机调节运动负荷量进行运动训练,第1实验组(n=20)在15°倾斜度和12m/min的速度下运动,第2实验组(n=20)在30°倾斜度和24m/min的运动速度下运动,另一组(n=20)为对照组。两实验组在神经损伤后第2周开始行25min/d,共3周的运动训练,分别测定倾斜台上维持姿势的最大角度,坐骨神经功能指数(SFI)行动学检查及神经传导速度。结果:在倾斜台上能保持原有姿势的最大角度,两实验组(85.0±1.0)°,(85.1±1.3)°均比对照组(75.8±2.2)°增高(P<0.05),但两实验组之间差异无显著性意义(P>0.05)。第2实验组的SFI值(-14.1±1.3)%比第1实验组的SFI值(-19.1±1.0)%减少(P<0.05)。两实验组的潜伏期(119.6±8.1)%,(118.7±3.6)%均比对照组(167.2±5.8)%缩短(P<0.05),但两组间无差异,损伤后的第4周第2实验组的波幅(50.6±1.9)%比另一实验组(45.7±2.2)%增高(P<0.01)。结论:坐骨神经受损伤大白鼠进行运动训练能促进其运动功能的恢复。在神经再生时期。

AIM:To investigate the effect of exercise training on functional recovery in animal models after peripheral nerve injury. METHODS:The experiment was completed in Central Laboratory,the Affiliated Hospital of Yanbian University College of Medicine.After the sciatic nerves were separated from 60 white male Wistar rats,hemostatic forceps were used to crush the position anterior to the branches of tibial and peroneal nerves for establishing models of crush injury of sciatic nerve.The 60 rats were divided into three groups,two experiment groups and a control group with 20 rats in each group and forced to exercise on the treadmill especially prepared for animals.Animals in the first experiment group exercised on the treadmill with 15°gradient at 12 m/minute(15°group),30°gradient at 24 m/minute in the second experiment group(30°group).Exercise training was given to the two experiment groups for 25 minutes daily after 2 week peripheral nerve injury for 3 weeks.Then,the maximal angle on the inclined board that the rat could maintain its initial position,behavioral examination for sciatic function index(SFI) and nerve conduction velocity were determined. RESULTS:The maximal angle on the inclined board that the rat could maintain its initial position in the two experiment groups[(85.0±1.0)°and (85.1±1.3) respectively] was bigger than that in the control group[(75.8±2.2)°](P< 0.05),but it was not of significant difference between the two experiment groups(P >0.05).SFI in the 30°group[(-14.1 ±1.3)%] was increased,as compared that in the 15°group[(-19.1 ±1.0)%](P< 0.05).The latency of the compound muscle action potentials(CMAP) in the two experiment groups[(119.6±8.1)%and (118.7±3.6)%respectively]was shorted,as compared with that in the control group[(167.2±5.8)%](P< 0.05),but it was no significantly different in the two experiment groups.On week 4 after injury,the amplitude of the CMAP was increased in the 30°group[(50.6±1.9)%],as compared with the 15°group[(45.7±2.2)%](P< 0.01). CONCLUSION:The exercise training for rats after sciatic nerve injury helps to improve the recovery of motor function,which is also accelerated with more exercise load during neural regeneration.