三七总皂甙对淀粉样β_(25～35)肽诱导的NG108-15细胞老年性痴呆模型的保护作用(英文)

Protection of panaxnotoginsengsaponins against NG108-15 cells in senile dementia model induced by amyoid beta-peptide 25-35

背景:阿尔茨海默病(Alzheimerdisease,AD)的病理病因虽然还没有完全清楚,但目前认为β-淀粉样肽(amyloidβ-peptide,Aβ)是引起AD的胆碱能神经功能紊乱的主要成分。因此,Aβ的产生及其对神经细胞的影响,以及寻找该病的发病机制、治疗方法及其有效的治疗药物成了医药界的研究热点。目的:观察三七总皂甙(PNS)对NG108-15细胞老年性痴呆模型的影响,为寻找该病的发病机制提供佐证,为研究和开发中药的新成分提供理论依据。设计:体外平行对照实验。单位:广西中医学院新药开发中心,广西中医学院生物化学教研室。对象:实验在广西中医学院新药开发中心完成。NG108-15细胞:中国科学院上海细胞研究所提供。PNS:云南玉溪维和制药厂生产。Aβ25～35片段:德国Sigma公司产品,批号:042K49501。MTT(四甲基噻唑蓝),德国Sigma公司产品,批号:020609。cAMP(环一磷酸腺苷)日本ャマサ酱油株式会社产品,批号:00207。胚胎牛血清(FBS):由美国Hy-clone公司提供,批号:0405。DMEM培养基:美国GIBCO公司产品,批号:0311。干预:NG108-15细胞培养法:含50mL/L胚牛血清、10g/LHAT、10g/L青霉素和链霉素(1:1)的DMEM培养液,置100mL/LCO2、37℃用的培养箱内培养殖,每隔一天换一次液,4d传代一次。主要观察指标:利用MTT法、细胞计数法和细胞形态学?

BACKGROUND:Although the pathology and etiology of Alzheimer disease(AD) have not been clear completely yet,amyloid β peptide(Aβ) is known as the main component induced cholinergic neural functional disturbance in AD.Therefore,the hot topics on researches in the medical field are focused on Aβproduction and its influence on neural cells and searching for the mechanism on the onset of disease,therapeutic methods and the effective therapeutic medications. OBJECTIVE:To observe the influence of panaxnotoginsengsaponins(PNS) on NG108 15 cells in senile dementia model,to provide evidences for searching for the mechanism on onset of disease and provide theoretic basis for researching and developing the new components of Chinese herbs. DESIGN: Parallel controlled trial in vitro. SETTING: Developing Center of New Drugs of Guangxi University of Chinese Medicine,Teaching Research Room of Biochemistry in Guangxi University of Chinese Medicine. PARTICIPANTS: The experiment was accomplished in Developing Center of New Drugs of Guangxi University of Chinese Medicine.NG108 15 cells:provided by Shanghai Cellular Institute of China Scientific Academy.PNS:produced by Yunnan Yuxi Weihe Pharmaceutical Factory.Aβ25-35 segments:the product from German Sigma Company,No. 042K49501.MTT:the product from German Sigma Company,No.020609.cAMP:the product from Japanese ャマサSoy sauce Company,No.00207.FBS:provided by American Hyclone Company,No.0405.DMEM medium: the product from American GIBCO Company,No.0311. INTERVENTIONS:NG108 15 cellular cultivation: DMEM culture fluid contained 50 mL/L FBS,10 g/L HAT,and 10 g/L penicillin and streptomycin(1∶1) was placed in a culture box of 100 mL/L CO2,at 37 ℃for cultivated proliferation.The solution changed once every other day and the generations passed down once every 4 days. MAIN OUTCOME MEASURES:To observe the changes of PNS on survival rates and process growth of NG108 15 cells determined by MTT method,cell counting methods and cytomorphology. RESULTS:PNS can increase ,by the neural toxicity of Aβ25-35 segments, the survival rate of NG108 15 cells [proliferation:(311±21)%,differentiation :(113±31)%,P< 0.05,F=5.567],the process growth rate[proliferation:(25.66±3.65)%,differentiation:(67.97±2.47)%,P< 0.05,F=6.471] and the average length of cell process growth [proliferation:(25.66±3.09) μm,differentiation:(25.66±3.65) μm,P< 0.001,F=12.641]. CONCLUSION:PNS can minimize the neural toxic reaction of Aβto cells and promote the growth of cellular process,indicating the antagonism of PNS to the pathological development of senile dementia.