刺五加皂甙对大鼠血管性痴呆的防治作用

Effects of acanthopanax senticousus saponins against vascular dementia in rats

目的:探讨刺五加皂甙对血管性痴呆(vasculardementia,VD)模型鼠学习记忆等认知功能的改善和对海马CA1区神经元的保护作用。方法:实验于2002-05/2003-05在南通大学航海医学研究所完成。选择SD大鼠36只随机分为对照组、模型组、用药组各12只,采用4-血管阻断改良法制备VD大鼠模型,用药组术前1周开始腹腔注射刺五加皂甙100mg/kg,术后再用1周,模型组腹腔注射同量生理盐水。各组作避暗回避试验及透射电镜观察。结果:行为学测试表明造模后1周模型组动物[(3.7±0.8)次]较对照组[(1.5±0.4)次]的探索次数明显增多(P<0.01),而滞留时间[模型组(195±5)s,对照组(995±8)s]显著缩短(P<0.01);用药组[(2.9±0.5)次]与对照组相比探索次数增多(P<0.01),但仍低于模型组(P<0.05),而滞留时间[(263±7)s]与对照组相比明显缩短(P<0.01),但仍高于模型组(P<0.05)。形态学结果表明刺五加皂甙减轻海马CA1区神经元损害。结论:刺五加皂甙能减轻海马脑缺血缺氧后神经元损害,从而改善VD大鼠学习记忆功能。

AIM:To study the effects of Acanthopanax Senticousus Saponins(ASS) for improving cognitive function and protecting neurons in hippocampal CA1 region of vascular dementia(VD) model rats. METHODS:The experiment was completed in the Institute of Navigation Medicine,Nantong University between May 2002 and May 2003.SD rats were selected and randomly divided into control group,VD model group and ASS group with 12 rats in each group.Rat model of VD was established by using 4 vessel occlusion method. Intraperitoneal injection of ASS at a dosage of 100 mg/kg was performed before operation and after operation for 1 week separately.The rats in the model group were intraperitoneally injected with the same volume of normal saline.Step down test was carried out,and the morphologic changes in CA1 region of hippocampus in the three groups were observed under transmission electron microscopy. RESULTS:The behavioral tests indicated that one week later, the exploring times in the VD group[(3.7±0.8) times] were significantly increased as compared with the control group[(1.5±0.4) times](P< 0.01),but staying time was significantly shorter in the VD group as compared with the control group.The exploring times in the ASS group[(2.9±0.5) times] were more than that in the control group(P< 0.01),but lower than that in the VD group(P< 0.05).Compared with the control group, the staying time was significantly shortened in the ASS group (P< 0.01), but it was longer than the VD group(P< 0.05).The morphology demonstrated that ASS could relieve neuron lesion of hippocampal CA1 region. CONCLUSION:ASS can relieve the damage to the neurons after hippocampal ischemia hypoxia, so as to improve the learning and memory of VD rats.