含雌激素中药脑保护作用的实验研究

Experiment on brain protection of herbal-estrogen

目的:探讨含雌激素中药对实验动物缺血后脑损伤的保护作用及其临床意义。方法:实验于2004-03/09在南京大学医学院附属鼓楼医院神经科实验室进行。将清洁级SD大鼠30只随机分为两组各15只,分别灌注中药和饮用水7d后,制成缺血再灌注脑损伤模型。在再灌注12,14h时每组各取6只取脑切片,TTC染色,计算梗死面积。另3只取缺血侧皮质,提取蛋白,并作雌激素受体β测定。结果:中药组脑梗死面积再灌注12h时为(40.62±1.68)%、再灌注24h时为(58.89±2.12)%,与对照组[(5.23±1.23)%和(69.86±2.43)%]相比,差异有显著性意义(P<0.05);免疫印迹蛋白测定,中药处理组缺血侧脑皮质雌激素受体表达高于空白对照组。结论:含雌激素中药有明显的脑保护作用。可能通过激活雌激素受体β而具有脑保护作用。

AIM:To investigate the protective effect of herbal estrogen against post ischemia brain injury in experimental animals and its clinical significance. METHODS:The experiment was done in the Laboratory,Department of Neurology,the affiliated Drum Tower Hospital,the Medical College of Nanjing University from March to September 2004.Thirty SD rats,grade clearness,were randomized into two groups with 15 in each group.The rats in the two groups were intragastrically administrated with herb or drinking water respectively 7 days before the establishment of cerebral ischemia reperfusion models.After 12 and 24 hour reperfusion,six rats were selected in the each group and the brains were taken out to make sections stained with TTC,and infarct area was calculated.Ischemic cortex was taken from another 3 rats in each group to extract protein, and estrogen receptor(measurement was performed. RESULTS:The infarct area of herbal estrogen group[(40.62±1.68)%and(58.89±2.12)%] was smaller than that of control group[(5.23±1.23)%and(69.86±2.43)%] after 12 and 24 hour reperfusion(P< 0.05). Western blot showed that the expression of estrogen receptor in ischemic cortex was higher in the herbal estrogen group than the control group. CONCLUSION:Herbal estrogen has an obvious role in protecting brain,which may result from the activation of estrogen receptor.