摘要
试验采用3个马铃薯栽培品种为材料,取2～12mm的花蕾,用醋酸—洋红压片法制片,在普通研究显微镜下观察花粉发育状况。试验结果表明,3mm以下的花蕾,花粉发育基本处在二分孢子和四分孢子时期,花蕾4～5mm大小时为花粉发育的单核高峰期,开花授粉前,大部分花粉为双核花粉,三核花粉的比例很小。处在发育早期的花粉均属正常,随着花粉发育进入到二核期以后,花粉畸形比例显著增加。品种间畸形花粉率存在显著差异。
Three varieties of S.tuberosum were used in the experiment. The flower buds of 2～12mm were taken from field and pollen grains were stained with aceto carmine and their development stages were observed under optical microscope. The results showed that most of pollens from the buds less than 3mm were in the stages of dyad and tetrad. The peak of single-nucleus pollen appeared when buds had a length of 4～5mm and most of pollens developed into double-nucleus stage just before blossoming. At this time, the proportion of trinuclcate pollen was low. Pollens were normal when they were in the early stage of development but the proportion of deformed polloen increased remarkblely while pollens developed into double-nucleus stage and thereafter. However, an obvious variance was observed among varieties.
出处
《马铃薯杂志》
1993年第4期202-205,共4页
Chinese Potato Journal
