木瓜凝乳蛋白酶的分离纯化及体外溶核作用

Purification of Chymopapain and Its Effect on Nucleus Pulposus in Vitro

目的 :建立简单有效的木瓜凝乳蛋白酶分离纯化方法 ,并研究其体外溶核作用。方法 :采用离子交换层析直接将木瓜凝乳蛋白酶分离纯化 ,体外试管法测定该酶的溶核作用。结果 :建立了简单有效的木瓜凝乳蛋白酶分离纯化方法 ,其纯化产品能溶解人体椎间盘的髓核。结论 :直接用离子交换层析柱来分离纯化木瓜凝乳蛋白酶是可行的 。

Objective:To establish a simple and effective procedure for purification of chymopapain and study about its effect on nucleus pulposus in vitro.Methods:Chymopapain was purified by ion exchange chromatograph and tested its effect on nucleus pulposus in vitro.Results:A simple and effective procedure for purification of chymopapain was established and the chymopapain did degrade nucleus pulposus.Conclusion:The ion exchange chromatograph was a simple and effective procedure for purification of chymopapain.It is necessary to study its effect on nucleus pulposus in vivo.