蛇葡萄素对人肺癌GLC-82裸鼠移植瘤的抑制作用

Anti-Tumor Effects of Ampelopsin on Human Lung Cancer GLC-82 Implanted in Nude Mice

目的 :为了进一步研究蛇葡萄素体内抗肿瘤作用 ,我们进行了人肺癌GLC 82裸小鼠移植瘤的抑瘤实验研究。方法 :人肺癌GLC 82细胞接种于裸鼠腋窝建立移植模型。荷瘤BALB/C裸鼠随机分成 6组 ,蛇葡萄素以 3个剂量腹腔给药。观察肿瘤体积、相对肿瘤体积、瘤重、相对肿瘤增殖率以及肿瘤生长曲线 ,以此评价蛇葡萄素的抗瘤作用。结果 :2 5 0mg/kg蛇葡萄素对裸鼠移植瘤的抑制率 ,二次实验结果分别为 35 5 % (P <0 0 1)和 37 1% (P<0 0 1) ,相对肿瘤增殖率则分别为 5 5 2 4 % (P <0 0 1)和 5 7 71% (P <0 0 5 )。结论 :蛇葡萄素对人肺癌GLC 82裸鼠移植瘤具有显著的抑制作用。

Objective:To investigate the effects of ampelopsin on the growth of human lung cancer cell line GLC-82 in nude mice.Methods:GLC-82 cells were inoculated into the armpit of nude mice to establish lung cancer model.Then the BALB/C nude mice with xenograft tumor were randomized into 6 groups.Ampelopsin was administered at 3 dosages by intraperitoneal injection daily.The anti-tumor effect of ampelopsin was evaluated on tumor volume,relative tumor volume,tumor weight,relative tumor proliferative rate,and tumor growth curve.Results:At the 250 mg/kg dose of ampelopsin,the growth inhibition of the transplant tumor was 35.5%(P<0.01) and 37.1%(P<0.01),and the relative tumor proliferative rate was 55.24%(P<0.01) and 57.71%(P<0.05),respectively in two independent experiments by compared with the normal saline control.Conclusion:Ampelopsin appears to be a potent anti-tumor agent that was firstly discovered in the transplant human lung cancer cell line GLC-82 in nude mice in vivo.