应用大孔吸附树脂吸附分离技术制备菟丝子总黄酮的研究

Study on Enrichment Process of Total Flavones from Semen cuscutae with Macroporous Resin

目的 :研究大孔树脂富集菟丝子药材总黄酮的工艺条件和参数。方法 :以总黄酮为指标 ,以比色法测定含量 ,考察富集总黄酮工艺条件。结果 :菟丝子提取液回收乙醇后上大孔树脂柱 ,水洗除杂质 ,以 30 %及 70 %乙醇洗脱 ,为最佳工艺。结论

Objective: To study the optimal conditions and parameters for the enrichment process of total flavones in Semen cuscutae with macroporous resin. Methods: With the enrichment degree of total flavones in Semen cuscutae as an index, the optimal conditions of the enrichment process were investigated. Results: The aqueous extract from Semen cuscutae was taken into the macroporous resin column, then the resin was washed by distilled water to get rid of impurity and 30% and 70% ethannol to elute total flavones. The above conditions were optimal. Conclusions: The process is feasible to enrich effective ingredient flavones from Semen cuscutae.