Ki-67、早期凋亡相关蛋白M30在子宫内膜癌的表达及其与预后的关系

Study on expression of Ki-67, early apoptotic protein M30 in endometrial carcinoma and their correlation with prognosis

目的 探讨子宫内膜癌增殖抗原Ki 6 7及早期凋亡相关蛋白M 30 (M30CytoDEATH ,细胞角蛋白 18)的表达与其生物学行为间的关系。方法 采用免疫组织化学链霉素抗生物素蛋白 过氧化物酶 (SP)法 ,分别检测了 79例子宫内膜癌Ki 6 7及M30蛋白的表达情况。结果 79例子宫内膜癌中 ,不同病理学分级间Ki 6 7平均指数分别为 :G1:2 0 4 8± 14 86 ;G2 :2 4 12± 14 4 2 ;G3:38 84± 11 88。不同临床分期间Ki 6 7平均指数分别为 :Ⅰ期 :2 0 6 5± 13 5 6 ;Ⅱ期 :2 6 92± 14 71;Ⅲ期 :35 14± 14 70。不同病理学分级间M30蛋白平均指数分别为 :G1:1 0 3± 1 4 2 ;G2 :1 0 3± 1 6 4 ;G3:1 94± 1 2 0。不同临床分期间M30蛋白平均指数分别为 :Ⅰ期 :0 30± 0 5 8;Ⅱ期 :1 6 6± 1 74 ;Ⅲ期 :2 0 7± 1 6 2。研究结果还显示子宫内膜癌M30指数随Ki 6 7指数增高而增高 ,它们之间呈显著正相关。生存分析表明 ,Ki 6 7指数≥ 30、M30指数≥ 2的患者比Ki 6 7指数≤ 10、M 30指数≤ 1的患者生存率显著降低。结论 Ki 6 7指数、M 30指数越高 ,子宫内膜癌生物学行为越差 ,患者预后也越差。Ki 6 7指数、M 30指数较高的患者 ,生存率显著降低。高分化子宫内膜癌 (G1)中 ,Ki 6 7指数≥30、M30指数≥ 2者可能提示患者预?

Objective To assess the correlation between expression of proliferation antigen Ki-67,early apoptotic protein M30 (M30CytoDEATH, CK18) and biologic characteristics in endometrial carcinoma. Methods SP immunohistochemical technique was used to detect the expression of Ki-67 and M30 in 79 cases of endometrial carcinoma respectively. Results The mean Ki-67 indices varied with histological grading and clinical stage of the tumor, being 20.48±14.86 in grade 1, 24.12±14.42 in grade 2 and 38.84±11.88 in grade 3; 20.65±13.56 in stage Ⅰ; 26.92±14.71 in stage Ⅱ; and: 35.14±14.70 in stage Ⅲ. The mean M30 indices varied with the grading and stage of the tumor, being 1.03 ±1.42 in grade 1, 1.03±1.64 in grade 2 and 1.94±1.20 in grade 3; 0.30±0.58 in stageⅠ; 1.66±1.74 in stageⅡ; and 2.07±1.62 in stage Ⅲ. Conclusions Ki-67 and M30 indexes are significantly correlated with biologic behavior and prognosis in endometrial carcinoma. There is a positive relationship between Ki-67 and M30 indexes.