套细胞淋巴瘤石蜡包埋组织中细胞周期蛋白D1和t(11;14)易位检测的临床病理意义

Detection of cyclin D1 protein expression and t (11;14) chromosomal translocation in paraffin-embedded tissues and its clinicopathologic significance for mantle cell lymphoma

目的 探讨套细胞淋巴瘤石蜡包埋组织中细胞周期蛋白 (cyclin)D1和t(11;14 )易位检测的可行性及其诊断和鉴别诊断价值。方法 收集套细胞淋巴瘤 36例 ,对照组小B细胞恶性淋巴瘤71例 ,均为石蜡包埋组织 ,运用免疫组织化学方法观察cyclinD1的表达 ;用半巢式聚合酶链反应(PCR)法检测t(11;14 )易位 ,以看家基因 β 肌动蛋白 (actin)作为内对照检测DNA质量。 结果 (1)36例套细胞淋巴瘤中 2 6例 (72 2 % )表达cyclinD1,对照组无 1例表达。 (2 ) 10 7例标本中 10 1例(94 4 % )可检出 β actinDNA表达。 36例套细胞淋巴瘤中 2 2例检出t(11;14 )易位 ,对照组无 1例检出。去除 β actin和t(11;14 )易位均阴性 2例 ,套细胞淋巴瘤中t(11;14 )易位检出率为 6 4 7%。 (3)36例套细胞淋巴瘤中cyclinD1染色和 (或 )t(11;14 )易位检测阳性病例为 2 9例 ,总阳性率为 80 5 %。结论 套细胞淋巴瘤石蜡包埋组织中cyclinD1和t(11;14 )易位的检测具较高的特异性和可行性 。

Objective To investigate the feasibility of detecting cyclin D1 protein expression and t(11;14) chromosomal translocation in paraffin-embedded tissues and its diagnostic and differential diagnostic significance for mantle cell lymphoma (MCL). Methods Paraffin-embedded samples of 36 cases of MCL and a control group of 71 cases of small B-cell lymphomas were retrieved from archive materials. Immunohistochemical staining for cyclin D1 and semi-nested PCR for t (11;14) were detected in all samples. House-keeping gene β-actin was used to detect the quality of DNA. Results (1) Cyclin D1 was expressed in 26 of the 36 MCL (72.2%). There was no cyclin D1 expression in the control group. (2) β-actin DNA was detected in 101 of the 107 tumor cases (94.4%). t (11;14) was detected in 22 of the 36 MCL. Translocation was not found in control group. The positive rate for t (11;14) was 64.7% in MCL after exclusion of 2 cases which were negative for both t(11;14) and β-actin. (3) 29 cases were positive for cyclin D1 and/or t (11;14), the positive rate reached 80.5%. Conclusion The combined detection of cyclin D1 and t (11;14) in paraffin-embedded tissues is found to be a specific and feasible method for diagnosis and differential diagnosis of mantle cell lymphoma.