体外肿瘤细胞对血管内皮细胞影响的观察

Influence of tumor cells on endothelial cells in vitro

目的 观察肿瘤细胞与血管内皮细胞的关系及其在体外直接接触培养时内皮细胞的形态变化。方法 免疫磁珠阳性选择法分离培养大鼠肺血管内皮细胞 ,生长至汇合状态时加入不同的肿瘤细胞 (小鼠树突状细胞肉瘤细胞DCS、人胃癌细胞MGC 80 3、小鼠肺腺癌细胞LA795 )共培养 ,光镜、扫描电镜、免疫组织化学、transwell法及荧光分子传递等方法观察内皮细胞的形态、功能的变化。结果 培养的内皮细胞部分呈卵石样 ,部分呈长梭形。长至汇合状态的内皮细胞再加入肿瘤细胞后 ,内皮细胞形态发生明显变化 ,汇合细胞中间出现许多圆形管腔样结构 ;肿瘤细胞往往分布在新形成的腔隙内 ;肿瘤细胞条件培养基可支持内皮细胞的生长 ,促进内皮细胞的运动 ;lucifferyellow分子可直接从肿瘤细胞传递到内皮细胞。结论 肿瘤细胞与内皮细胞可进行通讯 。

Objective To observe in vitro changes of endothelial cells after confrontation with tumor cells. Methods Dynobeads were used to isolate the endothelial cells from the rat lung. Mouse dendritic cell sarcoma cells (DCS), human gastric carcinoma cells (BGC-823) and mouse lung adenocarcinoma cells (LA795) were added to the endothelial cells when the latter was at the confluence phase. Phase contrast microscope, scanning electro-microscope, immunohistochemistry, transwell and fluorescence dye transfer were used to detect morphological and functional changes of the endothelial cells. Results Endothelial cells may look like cobble stones or long spindle shaped. Direct contact of tumor cells with endothelial cells induced round vascular-like space formation between confluent endothelial cells. Tumor cells were often found at the newly appeared spaces. Tumor cell conditioned medium could support the growth and promote the locomotion of endothelial cells through transwell. It was observed that luciffer yellow was directly transported from tumor cells to endothelial cells. Conclusions Tumor cells can directly induce morphological and functional changes in endothelial cells. Direct intercellular communication between tumor cells and endothelial cells is present.