前列腺组织雄激素受体的测定方法

ANDROGEN RECEPTOR ASSAY IN HUMAN PROSTATE TISSUE

建立雄激素受体测定的放射配基结合分析法测得人前列腺组织中胞浆胞核雄激素受体均可被约5x10^(-9)mol/L的双氢睾酮饱和,其最大结合客量(B(-max))及平衡解离常数(Kd)分别为:胞浆129.4x10(-15)mol/mg,0.88x10(-9)mol/L;胞核176.2x10^(-15)mol/mg,3.2x10^(-9)mol/L。人前列腺癌胞浆、胞核雄激素受体均高于正常前列腺(n=3)及前列腺肥大者(n=32)。用融裱法放射自显影证实雄激素受体主要分布在前列腺组织的腺区,尤其是在腺上皮细胞核内。

In this laboratory, to determine androgen receptor, the authors established RBA (radioligand binding assay of receptor) method. The study demonstrated that in the prostate both cytoplasmic and nuclear androgen receptor (AR) can be saturated by 5×10-9 mol/L dihydrotestosterone (DHT), the value of Bmax and Kd are 129.4×l0-15 mol/mg and 0.88×10-9 mol/L in cytoplasmic, 176.2 × 10-15 mol/mg and 3.2×10-9 mol/L in nucleus. Both the cytoplasmic and nuclear AR content in prostatic cancer are higher than those in normal prostatic and benign prostate hypertrophy (P<0.05). In addition the authors use thaw-mount autoradiography to confirm the epithelia cells of the glandular tissue of prostate, which are the target cells of AR.