原发性肝癌患者血清多种微量元素、铜蓝蛋白、T淋巴细胞测定与分析

^Determination of Element Contents, Caeruloplasmin, and T Lymphocites in the Serum of Patients with Carcinoma of Liver and Their Clinical Significance

应用ＩＣＰ－ＡＥＳ、酶氧化反应、酸性非特异性酯酶方法，对３３例原发性肝癌患者，测定血清多种微量元素、铜蓝蛋白及Ｔ淋巴细胞，分别与１７例、３６例、３４例健康者对照．原发性肝癌组血清铜ｘ±ｓ＝１９．１９±６．７０８μｍｏｌ／Ｌ）高于对照组（ｘ±Ｓ＝１４．８２±４．３７μｍｏｌ／Ｌ），血清钼（０．４３７±０．３１２μｍｏｌ／Ｌ）、锌（１５．５１±８．７７μｍｏｌ／Ｌ）、铁（２０．９７±２６．１３μｍｏｌ／Ｌ）均低于对照组铝（１．１８６±０．３１２μｍｏｌ／Ｌ）、锌（２０．９４±６．３１μｍｏｌ／Ｌ）、铁（４６．７２±２９．８９μｍｏｌ／Ｌ），Ｐ值均＜０．０５；肝癌组铜蓝蛋白（ｘ±Ｓ＝５．３８±２．３５活性单位／ｍＬ）高于对照组ｘ±Ｓ＝３．９２±０．８７活性单位／ｍＬ，Ｐ＜０．０５；Ｔ淋巴细胞（ｘ±Ｓ＝５７．７８±１１．７１活性单位／ｍＬ），低于对照组（ｘ±Ｓ＝６６．８０±１２．８２活性单位／ｍＬ），Ｐ＜０．０５．结果提示原发性肝癌微量元素代谢异常，直接影响酶系统，使自由基失控、机体内促氧化和抗氧化平衡失调，估计这一学说在肝癌的发病中起重要作用。

Contents of trace elements (Mo, Zn, Cu, Fe, Ba, Cr, Mn, Sr, Ta, Cd, Ni, Bi), caeruloplasmin and T lymphocites in the serum of 33 liver carcinoma patients with no complications or serious co-existing diseases have been determined by ICP-AES, enzyme oxidation reaction,and acid α-naphthyl acetale esterase methods, separately contrasted with 17, 36 and 34 cased of normal persons. The results show:Normal contrasts (x±SD) Mo 1. 186±0. 312, Zn 20. 94± 6. 51, Fe 46. 72±29. 89, Cu14. 82 ± 4. 37μmol/L; Caeruloplasmin (x ±SD ) 3. 92 ±2. 51 activity units/mL; Tlymphocites (x±SD) 66. 80±12. 82.Liver carcinoma patients (x±SD) Mo 0. 437±0. 312, Zn15. 51±8. 778±29. 89, Fe20. 97±26.13, Cu 19.19±6. 708μmol/L; Caeruloplasmin (x ±SD) 5.38±2. 35 activity units/mL; T lymphocites (x ±SD) 57. 78±11. 71; Cu serum and caeruloplasmin are positively related, γ= 0. 706,P<0. 001.The contents of Mo. Zn. Fe for liver carcinoma patients are remarkably lower than in the normal controls, P <0. 01 or P<0. 05 ; but Cu and caernloplasmin are remarkably highter than normal controls, P<0. 05. This suggests that the abnornal metabolism of trace elements in liver carcinoma directly affects the enzyme system causally loss to contral on the free radical, and loss of balance in oxidation metabolism, this mechanism will probably be of reference to the occurrence of liver carcinoma.