应用封闭式Dexsol基质保存角膜的研究

A study of corneal preservation with closed Dexsol medium

应用自制的 Dexsol 基质保存角膜,对40只动物(兔、猴各20只)角膜及5只人角膜进行了实验研究。保存时间为4℃者2周,34℃者5周。对保存在不同时间及不同温度的角膜应用锥蓝及茜素红双重染色,角膜内皮细胞活性良好。透射及扫描电镜观察保存2周(4℃)及4周(34℃)的角膜内皮细胞超微结构均无病理性改变。应用 Dexsol 基质保存的兔角膜进行同种穿透角膜移植,术后2个月,植片透明。20例接受 Dexsol 基质保存的供体角膜移植的患者,术后3个月移植片透明。植片中央部角膜内皮细胞密度平均为1915个/mm^2。

An experimental study was made on the preser- vation of donor corneas with a Dexsol medium prepared,a total of 40 animal corneas(rabbit and monkey,20 each)and 5 human corneas were used.The corneas were preserved separately at 4℃ for 2 weeks and at 34℃ for 5 weeks.The corneas preserved at different temperatures for different lengths of time were doubly stained with trypan-blue and alizarin red,which revealed that the endothelial cells remained fully active.Transmisscon and scanning election microscopy showed that neither the corneas preserved at 4℃ for 2 weeks nor those preserved at 34℃ for 5 weeks had any pathological changes in the ultrastructure of their endothelial cells.The rabbit cornea preserved with the Dexsol medium was used in homoplastic perforating keratoplasty,which resulted in a transparent transplant 2 months after the opera- tion.In 20 patients who received donor corneas preserved in the Dexsol medium for keratoplasty, the grafts remained clear 3 months after operation, and had an average endothelial cellular density of 1915/m^2 in the central region.