氢化物电热原子吸收法测定人血清硒

Determination of Selenium in Blood Serum by Hydride Atomic Absorption Method

氢化物原子吸收法是原子吸收分光光度法的一个重要分支．笔者应用氢化物电热原子化装置测定血清的硒具有用血量少、成本低、方法简便、精度佳等优点，适合一般医院开展科研和临床测定．采用这种技术可减少试样的基体干扰，提高了分析灵敏度，而且具有良好的线性关系，其测定检出限为１．２μｇ／Ｌ，浓度变异系数为６．３％。

The hydride atomic absorption method is an important branch of atomic absorption spectrophotometry. The selenium (Se) in blood serum is determined by the author with hydride electrothermal atommizing device. This method has many advantages such as less blood quantity demand. low cost. easy operation and high precision. and is suitable for scientific research and clinic determination at ordinary hospitals. This technique can adopted to reduce the matrix disturbance of specimen, increase analytical sensitivity and has good linear relation, Its detection limit is 1. 2μg/L. The coefficient of concentration variation is 6. 3%.