oxLDL对血管内皮细胞PECAM基因表达的影响

A Pilot Study on the Effect of oxLDL on PECAM Gene Expression in Endothelial Cells

培养人脐带静脉内皮细胞（ＥＣ），提取人血浆低密度脂蛋白（ＬＤＬ）并制成氧化型低密度脂蛋白（ｏｘＬＤＬ），分别与血管ＥＣ培养后提取各组ＲＮＡ，进行电泳及Ｎｏｒｔｈｅｒｎ印迹杂交，用人β－ａｃｔｉｎ基因探针和人ＰＥＣＡＭ基因探针与各组ＲＮＡ进行杂交。结果显示：ＥＣ在Ｅ－ＧＭＵＶ培养液中生长度好并可多次传代培养；用ｏｘＬＤＬ处理ＥＣ２４ｈ可导致细胞死亡；用一步法提取单层培养ＥＣ的ＲＮＡ效果较好；用地高辛标记人β－ａｃｔｉｎ基因探针与各组ＲＮＡ杂交均可见杂交带，而未见ＰＥＣＡＭ基因表达。提示单层培养人血管ＦＣ适于基因表达的研究；ｏｘＬＤＬ对血管ＥＣ有细胞毒性，但不能促进ＰＥＣＡＭ基因表达。

xidized low density lipoprotein(oxLDL) is one of important risk factors on atheroscle and atherosclerotic diseases.Moreover,Platelet endothelial cell adhesion molecule (PECAM) may induce rapid adhesion of platelets to endothelium that is one of the earliest events in atherosclerosis and inflammation.It was of interest to determine whether oxLDL may induce PECAM gene expression in endothelial cells.In this paper,cultured endothelial cells from human umbilical veins were treated with oxLDL or LDL,and total RNA were iso lated.After electrophoresis and Northern blot,hybridizations were done with human PECAM probe and β-actin probe.The results showed that the cells were grown well in EGMUV media and the cells were died after 24h treated with oxLDL but not injured after 6h treatment.In LDL treated group,the cells were less died in 24h.The hybridizative bands were found with β-actin probe hybridization.However,PECAM gene was not expressed.The results indicated that oxLDL may injure the endothelial cells but it can not induce PECAM gene expression of endothelial cells.