缺氧对海马培养细胞人重组白细胞介素－6免疫组化反应的影响

The Effects of Anoxia on Recombinant Human Interleukin-6 Immunoreactivity in Cultured Hippocampal Cells

本文观察了缺氧对体外培养海马细胞中ｒｈＩＬ－６免疫反应的影响。结果显示，体外培养的海马神经元和神经胶质细胞的ｒｈＩＬ－６呈弱阳性免疫反应，缺氧１～４ｈ后神经元和神经胶质细胞的ｒｈＩＬ－６免疫组化反应明显增强。对免疫染色细胞作图像分析结果显示，缺氧后神经元和神经胶质细胞的平均光密度较缺氧前增加，尤以缺氧２ｈ时最明显，之后随缺氧时间延长，神经元和神经胶质细胞的平均光密度又逐渐减弱。上述结果表明，海马脑区存在ｒｈＩＬ－６免疫反应细胞；缺氧条件下ｒｈＩＬ－６免疫组化反应增强，提示ｒｈＩＬ－６可能参与脑缺氧损伤的调控。

This study examined changes of recombinant human interleukin-6(rhIL-6)immunoreactivity in cultured hippocampal cells during anoxia. On day 12 after plating of the hippocampal cells from newborn rat,the cultures were exposed to anoxic atmosphere(90%N2+10% CO2)for 1-12 hours.The results showed that very light intensity of rhIL-6 immunoreacTivity(rhIL-6-IR)was detected in cell bodies and processes of both neurons and glial cells under normaxia.A rapid increase in rhIL-6-IR was found in both of neurons and glial cells after 1-4 h of anoxic exposure.The mean optical density of rhIL-6-IR assessed quantitatively by image analysis were significantly higher in anoxic cells,especially after 2 h of anoxia,than those in controls.Following progressive anoxia,increased rhIL-6-IR gradually returned to basal levels at 12 h after anoxia,These results demonstrate the cellular source of interleukin-6 produced in hippocampus. The early increase in rhIL-6-IR during anoxia suggests that interleukin-6 may participate in modulation of anoxic injury in brain.