IL—2基因修饰鼠肝癌细胞引起的凋亡研究

Studies on the Apoptotic Cell Death of Interleukin-2 Gene Modified Mouse Hepatoma Cells

用重组人白细胞介素-2(IL—2)含信号肽.DNA的缺陷型逆转录病毒将人IL—2基因整合于鼠肝癌细胞株H22染色体后,PCR及RT—PCR方法确证有人IL—2基因存在及表达,流式细胞仪检测表明IL—2基因修饰后的H22细胞中DNA低含量细胞明显多于对照组.用透射电镜分别观察了IL—2基因修饰H22细胞和未经修饰H22细胞的超微结构,发现IL—2基因修饰H22细胞其染色体有固缩等凋亡现象发生,说明人IL—2基因在鼠肝癌细胞中的表达具有促使肿瘤细胞发生凋亡的作用;这可能也是IL—2基因修饰后其致瘤性下降的重要机制之一.

Retroviral vector was used to introduce the interleukin - 2 (IL -2) gene into H22 cells, a BALB/c mouse hepatoma cell line. The IL-2 gene and marker gene (NeoR) were assessed by using PCR and RT-PCR methods. FACS analysis demonstrated that the cells with low DNA content in IL-2 gene modified H22 cells were more than these in control H22 cells. Electron microscopic morphological structure showed that some cells in IL-2 gene modified H22 went into apoptotic cell death. The study demonstrated that apoptotic cell death of H22 - IL-2 cells was induced by the IL-2 gene modification. It may be one of mechanisms of decreased tumorigenicity of IL - 2 gene modified tumor cells.