皮炎煎剂对小鼠实验性接触性皮炎的疗效及作用机理研究

Efficacy of Piyan Decoction in Treating Mouse Experimental Contact Dermatitis: the Mechanism of the Efficacy

目的 探讨皮炎煎剂治疗湿疹皮炎疗效及对迟发型超敏反应作用机理。方法 用 2 ,4 -二硝氯苯 (2 ,4 -DNCB)对ICR小鼠皮肤致敏和诱发 ,制成实验性接触性皮炎模型。以中药制剂 (皮炎煎剂 1号、皮炎煎剂 2号 )作为治疗组 ,以氢化可的松琥珀酸钠为阳性对照组 ,以生理盐水为阴性对照组。对小鼠耳壳皮损严重度、耳壳增重值、耳壳皮损炎症细胞计数变化进行统计分析。结果 皮炎煎剂 1号、2号和氢化可的松对模型鼠红斑、水肿、渗出的接触性皮炎损害均有明显抑制作用 ,可减轻由于充血、水肿等炎症反应导致的局部皮损重量增加 ,减少皮损部位炎性细胞浸润。以上 3组的抗炎作用明显优于阴性对照组 (P <0 0 1)。皮炎煎剂 1号优于皮炎煎剂 2号 (P <0 0 5 )。结论 皮炎煎剂对迟发型变态反应引起的小鼠接触性皮炎具有明显抑制作用。

Objective To observe the efficacy of TCM drugs for treating eczematous dermatitis and to investigate the mechanism of their effects on delayed-type hypersensitivity.Methods The mouse model of experimental contact dermatitis was established by sensitizing the skin of ICR mice with 2,4-dinitrochlorobenzen (DNCB). The treating groups were given Piyan Decoction No.1 and No.2, respectively, the positive control group was given hydrocortisone sodium succinate, and the negative control group was given normal saline. Statistical methods were used to analyze the severity of skin injury of the auricular concha, the magnitude of the increase in the weight of the injured auricular concha, and the changes in the inflammatory cell count in the auricular concha with skin injury. Results Piyan Decoction No.1 and No.2 and hydrocortisone sodium succinate showed marked inhibitory effects on erythema, edema and infiltration in the model group, hence lowering the increase in the weight of the auricular concha due to focal skin damage caused by congestion and edema, and reducing the inflammatory cell infiltration at the site of skin damage. The results had statistical significance, as compared with those in the negative group which was given normal saline (P<0.01). Furthermore, Piyan Decoction No.1 showed stronger effects than Piyan Decoction No.2 (P<0.05). Conclusion Piyan Decoction has marked inhibitory effects on the mouse contact dermatitis caused by delayed-type hypersensitivity.