人绒毛膜促性腺激素对重组E型选择素介导的细胞粘附的影响

Effect of Human Chorionic Gonadotropin on the HepG2 Cells Adhesion Mediated by Recombinant E-selectin

目的 :探讨孕妇羊水、血清和尿液的HCG对E型选择素介导的细胞粘附过程的影响。方法 :测定铬51标记的带有E型选择素配体—唾液酸化的Lewisx(sialyl Lewisx,sLex)结构的肝癌细胞系 ,即HepG2细胞在不同浓度、不同来源HCG存在下和重组E型选择素的粘附能力 ,从而评价HCG对E型选择素介导的细胞粘附过程的影响。结果 :血清和羊水HCG能抑制E型选择素介导的HepG2细胞的粘附作用 ,其抑制作用呈浓度依赖关系 ,相对抑制力分别为纯四糖结构sLex 的 2 4× 10 4和 4 5× 10 3 倍 ;而尿HCG无此作用。结论 :血清和羊水HCG是E型选择素的有效配体。

Objective: To explore the effect of HCGs isolated from human amniotic fluid, serum, and urine of pregnant women on cell adhesion mediated by E-selectin. Methods: The adhesion properties of 51 Cr-labelled HepG2 cells, which carry the ligands of membrane-bound sialyl-Lewis x epitope for E-selectin, were detected to recombinant E-selectin by means of adding different concentrations and sources of HCGs as potential inhibitors, and meantime, the effect of HCGs isolated from amniotic fluid, serum and urine in E-selectin mediated cell adhesion was assessed. Results: HCGs isolated from human amniotic fluid and serum of pregnant women inhibited the adhesion of HepG2 cells to E-Selectin in a dose-dependent manner, the relative inhibition was 2.4×10 4 and 4.5×10 3, respectively, compared with sialyl-Lewis x. But HCG isolated from urine of pregnant women did no effect. Conclusions: HCGs isolated from human amniotic fluid and serum of pregnant women is an effective ligand for E-selectin.