胰岛素对离体动脉平滑肌细胞钙化的影响

The Effects of insulin on calcium deposition of arterial smooth muscle cell calcification in vitro

目的 :动脉钙化是糖尿病患者血管疾病的主要病理改变之一 ,其原因不明。由于糖尿病的主要病理生理改变是机体组织细胞中胰岛素绝对或者相对缺乏 ,本研究试图探讨动脉平滑肌细胞在钙化过程中胰岛素的影响 ,以分析其在动脉钙化中的可能作用。方法 :建立牛离体动脉钙化的模型 ,培养钙化血管细胞 ,在培养环境中加入胰岛素 ,观察细胞钙沉积量的变化 ,同时分析钙化过程中细胞碱性磷酸酶活性和骨钙素 (OC)分泌的变化情况。结果 :胰岛素为 10 -8mol/L环境中 ,钙化血管平滑肌细胞的钙沉积与对照细胞相比差别不显著 ,但是胰岛素环境中的细胞钙化过程比较平缓。胰岛素组血管平滑肌细胞钙化过程中碱性磷酸酶活性在各个时间均显著低于对照组 (P <0 .0 1)。钙化细胞的OC分泌在胰岛素环境中有所降低 ,尤其在钙化中期更加明显 ,在第 4、6、8d胰岛素组的OC水平分别比对照水平降低了 4 8.7%、5 5 .1%和 2 7.7%。结论 :10 -8mol/L的胰岛素对动脉血管平滑肌的钙盐沉积影响不明显 ,但是能够显著降低钙化过程中碱性磷酸酶活性和OC分泌量 ,反映出胰岛素对血管平滑肌细胞的钙化过程有保护作用。

AIM: As one of the main pathological changes of diabetic melitius blood vessels, arterial calcification has always been an unknown process. The main cause of DM is the relative or absolute shortage insulin of tissue or cell. So it is rational to suppose that insulin has some role in the arterial calcification in DM patient. METHODS: β-glycerin-phosphate disodium salt was used to induce cultured bovine vessel smooth muscle cell to calcify which serves as a vascular calcifying model in vitro. Insulin were added to the culture media, made the final concentration to 10_-8mmol/L. After 10 days culture, the deposition of calcium, alkaline phosphatase(ALP) activity and osteocalcin(OC) concentration was quantified. RESULTS: After 10 days of culture, There is no significant change in calcium deposition in insulin media, but the calcified process is slightly gentle. The ALP activity and OC concentration in insulin media is much lower than control group. CONCLUSION: 10_-8mmol/L insulin didn′t influence the calcium deposition but lower the ALP activity and OC concentration, which indicate the protective effect on vascular calcification of smooth muscle cell.