银屑病易感基因位点的检测

Searching for the susceptibility loci of psoriasis in Chinese

目的 寻找中国人群银屑病的易感基因位点。方法 选取 19个多态性微卫星标记 ,采用由荧光标记PCR、MegaBACE测序仪和与之配套的GeneticProfiler分析软件建立的荧光标记的自动基因分型体系对 38个银屑病家系中的银屑病患者与亲属的外周血白细胞中提取的DNA进行基因扫描 ,并用参数和非参数分析方法进行连锁分析。结果 在参数连锁分析中 ,D6S16 10的最大两点连锁(LOD)值为 1 18(θ=0 2 ) ,D17S94 4的最大两点LOD值为 1 6 0 (θ =0 1) ;在非参数单点和多点连锁分析中 ,D6S16 10、D17S94 4和D17S785的单点非参数 (NPL)值均大于 1 6 ,相应P值也小于 0 0 5。结论 位点D6S16 10、D17S94

Objective To search for the susceptibility genes of patients with psoriasis in Chinese Methods 38 psoriasis families were determined to search for the susceptibility loci by selecting 19 reported polymorphic microsatellite DNA markers and making use of fluorescein labeled PCR with Mega BACE sequencer Results The significant two point Lod score values of parametric linkage analysis were obtained with D6S1610 marker(1 18 at θ=0 2) and D17S944 marker (1 60 at θ=0 1); the NPL values greater than 1 6 ( P <0 05) of non parametric analysis were obtained with D6S1610 marker, D17S944 marker and D17S785 marker Conclusion A putative linkage to D6S1610 marker, D17S944 marker and D17S785 marker