慢性食管炎和食管癌旁粘膜上皮癌基因蛋白表达的比较

A Comparative Study of Oncogene Protein Expression in Mucosa Epithelia of Chronic Esophagitis and Para-cancer

背景与目的:探讨慢性食管炎增生的粘膜上皮与食管癌旁粘膜上皮3种癌基因蛋白表达的关系。材料与方法:采用免疫组织化学EnVision二步法对47例慢性食管炎及85例食管癌和癌旁粘膜组织进行P53、P27和MDM2蛋白表达的检测。结果:P53蛋白在正常食管粘膜不表达,在食管炎、癌旁非典型增生,食管癌3组阳性率相似,高于正常食管上皮的阳性率;对于P27蛋白阳性反应,4组阳性率相似;MDM2在正常上皮、食管炎、癌旁非典型增生3组阳性率相似,低于食管癌的阳性率。慢性食管炎粘膜上皮P53、P27、和MDM2 3种蛋白表达与癌旁轻、中、重度非典型增生粘膜上皮相似。结论:慢性食管炎食管粘膜上皮P53、P27和MDM2 3种蛋白表达与癌旁粘膜上皮轻、中、重度非典型增生相似,提示慢性食管炎可能是一种癌前病变。

BACKGROUND & AIM: To explore the relationship of three proteins expression in esophageal mucosa epithelia between chronic esophagitis and esophageal para-cancer tissue. MATERIAL AND METHODS: Forty-seven cases of chronic esophagitis and 85 specimens with esophageal carcinoma and para-cancer tissue were analyzed by immunohistochemical staining for P53、 P27 and MDM2 proteins expression. RESULTS: The P53 protein expression was not detected in normal mucosa, the positive rates of three groups, esophagitis, dysplasia epithelia and cancer tissue are similar(X.2 = 0. 979, P = 0.0615) . From normal epithelium to chronic esophagitis and/ or dysplasia to esophageal carcinoma, the positive-reactivity for P27 protein were detected. The differences for the four groups have no statistically significance(X2= 1.828, P = 0.609) .While the positive rates of MDM2 protein have statistically significance in the four groups(X.2= 18.895, P = 0.000).And are similar in normal epithelium, chronic esophagitis and dysplasia epithelia(X2= 3.585, P = 0.167), and their positive rates are lower than esophageal carcinoma(X2 = 14.802, P = 0.301) . The expressions of P53、 P27 and MDM2 proteins are similar in chronic esophagitis and mild, moderate and severe dysplasia epithelia(P53、 P27, binomial distribution, minimum P = 0.2140; MDM2, X2= 3.658, P = 0.301) . CONCLIUSION: The expression of P53、 P27 and MDM2 proteins is similar in mucosa epithelia of chronic esophagitis and mild, moderate and severe dysplasia. This indicated that it is possible that chronic esophagitis is a kind of precancerous lesions.