大鼠前列腺的锥虫蓝透过性实验研究

Experimental Study on the Penetrability of Trypan Blue to the Rat Prostate

目的 :了解大鼠正常前列腺、炎症前列腺以及良性增生前列腺的锥虫蓝透过性。 方法 :取 6 0只SD雄性大鼠 ,随机分为正常组 (NP ,n =15 )、细菌性前列腺炎组 (BP ,n =15 )、良性前列腺增生组 (BPH ,n =15 )、良性增生合并细菌性前列腺炎组 (BPH BP ,n =15 ) ,各组随机抽取 5只作为不注射锥虫蓝的对照组 (NC ,n =5× 4 ) ,其余 10只分别由尾静脉注射 2ml1%锥虫蓝溶液。 2h后麻醉处死动物和分离前列腺 ,肉眼直接观察各组大鼠的前列腺被锥虫蓝染色情况 ,并用比色法检测前列腺组织的锥虫蓝浓度。 结果 :注射锥虫蓝的各组大鼠除脑组织和脊髓组织外 ,前列腺的表面及内部组织与肌肉、肝脏、肠道等组织同样被染成相似的蓝色。BP组和BPH BP组前列腺的锥虫蓝含量明显高于BPH组及NP组前列腺的锥虫蓝含量 (P <0 .0 5 )。 结论 :大鼠的正常前列腺、良性增生前列腺以及炎症前列腺都容易被具有较高分子量和离子化性质的锥虫蓝透过 ,但炎性前列腺具有更加明显增高的锥虫蓝透过性。

Objective: To understand the penetrability of trypan blue to the normal prostate as well as to the inflammatory prostate and the prostate with benign hyperplasia in rats. Methods: Sixty SD male rats were randomized into 4 groups: NP (normal prostate) group (n=15) , BP(bacterial prostatitis)group( n=15), BPH(benign prostatic hyperplasia)group(n=15), and BPH-BP(benign prostatic hyperplasia with bacterial prostatitis) group (n=15). Five rats were taken from each group as non-staining controls (NC, n=5×4) and the other 10 were injected by tail intravenation with 1% trypan blue and then the prostates were isolated from the rats killed by anaesthesia after 2 hours. The color of the prostates and other tissues of the animals were observed and the contents of the trypan blue in the tissues of the prostates were determined separately by colorimetry. Results: Apart from the tissues of brains and spinal cord the surface and the inner tissues of the prostates with NP, BP, BPH and BPH-BP from the rats injected with the dye were also dyed blue similar to that of the muscles, livers, intestines and others. The content of the trypan blue in the tissues of the prostates with NP, BP and BPH-BP was obviously higher than those with NP and BPH. Conclusion: The penetrability of trypan blue with properties of ionization and larger molecular weight is high in either the normal prostate or the prostate with BP, BPH and BPH-BP, and much higher in inflammatory prostate than in the normal prostate and the prostate with BPH.