不育症患者精浆IL-1β、IL-4、IL-10含量测定及临床意义

Determination of IL-1β, IL-4 and IL-10 Contents in the Seminal Plasma of Infertile Patients and Its Clinical Value

目的 :观察男性不育症患者精浆中白细胞介素 1β(IL 1β)、白细胞介素 4 (IL 4 )、白细胞介素 10 (IL 10 )含量 ,及其与精子的各项功能指标之间的相互关系。 方法 :应用放射免疫分析 (RIA)技术 ,对 12 6例男性不育症和 2 0例正常生育者精浆中IL 1β、IL 4、IL 10含量进行检测。根据精子密度将不育症患者分为A组 (精子密度≥ 2 0× 10 6/ml)、B组 (精子密度 <2 0× 10 6/ml)和C组 (无精子症者 ) 3组 ;根据精子活动力、活动率将A组分别分为精子活动力正常组和不良组 ,精子活动率正常组和下降组 ;根据不育症患者血清抗精子抗体 (AsAb)检测结果、精液中WBC多少分为AsAb阳性组和阴性组 ,WBC精液组和非WBC精液组。根据生育组检测结果 ,将不育A组和B组分为精子穿透力正常组和下降组 ,精子顶体完整率正常组和下降组 ,精子尾部肿胀率正常组和下降组。 结果 :不育症组精浆IL 1β含量显著高于生育组 (P <0 .0 1) ,IL 4、IL 10含量显著低于生育组 (P <0 .0 1)。不育症组精浆中IL 1β、IL 4、IL 10含量在WBC精液组与非WBC精液组、血清AsAb阳性组与阴性组之间差异均有显著性 (P <0 .0 5或P <0 .0 1) ;IL 4含量在不育症组精子活动力、活动率、精子穿透力、顶体完整率、尾部肿胀率正常与减少之间差异均有显著性 (P

Objective: To observe the relationship between the contents of interleukin-1β(IL-1β), interleukin-4(IL-4) and interleukin-10(IL-10) in the seminal plasma of infertile males and sperm function indexes. Methods: By radioimmunoassay (RIA),we determined the contents of IL-1β, IL-4 and IL-10 in the seminal plasma of 126 infertile and 20 normal males. Accoding to the sperm count, the infertile were divided into three groups: Groups A (sperm count≥20×10 6/ml), B (sperm count<20×10 6/ml) and C (azoospermia). Based on sperm vitality and motility, Group A was subdivided into a normal and abnormal vitality group and a normal and decreased motility group. In line with the serum results of antisperm antibody(AsAb) and semen WBC, the infertile males were divided into AsAb positive and negative, and WBC semen and non-WBC semen groups. According to the assay results of normal males, Groups A and B were each subdivided into normal and decreased groups of sperm penetrating power, intact acrosome rate and terminal swelling rate. Results: The content of IL-1β in the seminal plasma of the infertility group was obviously higher, but the content of IL-4, IL-10 significantly lower than that of the normal group(P< 0.01). In the infertility group, there existed significant differences in the contents of IL-1β, IL-4, IL-10 in seminal plasma between the WBC and non-WBC semen groups,as well as between the AsAb positive and negative groups(P< 0.05 or P< 0.01); and the same was true for the content of IL-4 between the normal and decreased groups of sperm vitality, motility, penetrating power, intact acrosome rate, and terminal swelling rate(P< 0.05 or P< 0.01). Conclusion: The contents of IL-1β, IL-4 and IL-10 in seminal plasma are closely related to male reproduction. The increase or decrease of the contents reflects the state of immunity and infection of the reproductive system, and influences sperm functions.