葛根素对实验性脑出血大鼠脑水肿及脂质过氧化的反应影响

Effect of puerarin on cerebral edema and lipid peroxidation in rat brain after experimental cerebral hemorrhage

目的 :探讨葛根素对脑出血的治疗作用及机制。方法 :采用立体定向胶原酶尾状核注射法制备动物模型。检测脑出血大鼠脑系数、脑含水量、脑组织超氧化物歧化酶 ( SOD)活性和丙二醛 ( MDA)含量的变化。结果 :与模型组比较 ,葛根素组的脑系数〔( 82 .9± 3.6 )× 10 - 4比 ( 86 .1± 3.3)× 10 - 4〕和脑含水量 ( 78.75 %±0 .5 7%比 79.99%± 0 .6 7% )均有显著下降 ( P均 <0 .0 1) ,脑组织 MDA含量明显降低〔( 5 4 .8± 6 .7) nm ol/ g比( 70 .2± 8.2 ) nmol/ g〕,而脑组织 SOD活性显著提高〔( 2 .32 4± 0 .0 90 ) k NU / g比 ( 1.2 93± 0 .0 73) k NU/ g〕,P均 <0 .0 1。结论

Objective: To investigate the therapeutic effects of puerarin on acute cerebral hemorrhage in rats and its mechanism. Methods: The rat model was established by injecting collagenase into the caudate nucleus of the rat with stereotaxic technique. The levels of superoxide dismutase(SOD) and malondialdehyde(MDA) in brain tissue, brain coefficient as well as the brain water content were detected. Results: Compared to the model rats, the brain coefficient and brain water content of the rats treated with puerarin were decreased〔(82 9±3 6)×10 -4 vs. (86 1±3 3)×10 -4 and 78 75%±0 57% vs.79 99%±0 67%, both P <0 01〕, the concentration of SOD in brain tissue was increased〔(2 324±0 090)kNU/g vs. (1 293±0 073)kNU/g, P <0 01 〕 but the concentration of MDA was decreased〔(54 8±6 7)nmol/g vs. (70 2±8 2)nmol/g, P <0 01 〕. Conclusion: The puerarin could lighten the degree of the cerebral edema of the rat cerebral hemorrhage model . It has the effect against the damage of lipid peroxidation and free radical.