葛根素对家兔心肌缺血-再灌注损伤的保护作用

Protective effects of puerarin on myocardial ischemia/reperfusion injury in rabbits

目的 :探讨葛根素抗心肌缺血再灌注损伤的机制。方法 :采用结扎冠状动脉左室支方法制备兔心肌缺血再灌注损伤模型 ,用四道生理记录仪测定动物的血流动力学及左室功能变化。将 4 0只家兔随机分组。10只作为空白对照组 ;10只作为假手术对照组 ;余 2 0只制模 ,其中 10只为模型组 ,另 10只则在结扎左室支前2 0 m in和再灌注开始时经静脉注入葛根素注射液作为保护组。分别在缺血 4 0 m in及再灌注 2 0 min时取静脉血测定脂质过氧化物 ( L PO)含量及超氧化物歧化酶 ( SOD)、谷胱甘肽过氧化物酶 ( GSH Px)活性 ;实验完毕后取缺血再灌注损伤区心肌组织 0 .5 g,分别测定组织匀浆中 L PO含量、SOD活性及 GSH Px/ L PO。结果 :与模型组比较 ,葛根素保护组动物左室内压峰值 ( L VSP)、左室内压上升最大速率 ( L V+ dp/ dt m ax)和左室内压下降最大速率 ( L V dp/ dt max)均显著升高 ( P<0 .0 5或 P<0 .0 1) ;血清中 L PO含量明显降低 ( P<0 .0 5 ) ,而GSH Px/ L PO升高非常明显 ( P<0 .0 1) ;缺血再灌注损伤区心肌组织中 L PO含量显著降低 ,SOD活性和GSH Px/ L PO均显著升高 ( P均 <0 .0 5 )。结论

Objective: To investigate the mechanism of puerarin against myocardial ischemia/reperfusion injury. Methods: The myocardial ischemia/reperfusion injury model of rabbits were reproduced by ligating the left ventricular anterior coronary artery, and by the fourchannel physiology recorder, the hemodynamic and the function of left ventricular were recorded. Forty rabbits were randomly divided into three groups: 10 animals were in the normal group , 10 animals were in the shamoperated control group ( n =10), and the other 20 animals reproduced myocardial ischemia/reperfusion injury models, which were randomly divided into model and puerarin subgroups. The puerarin group was injected puerarin injection intravenously at 20 minutes before ligating and at the beginning of reperfusion. Lipid peroxide (LPO), superoxide dismutase(SOD) and glutathione peroxidase(GSHPx) were detected at 40 minutes after ischemia and at 20 minutes after reperfusion . The ischemia/reperfusion injuried myocardial tissue of 0 5 g was sampled and LPO, SOD, GSHPx as well as GSHPx/LPO were detected in myocardial homogenate. Results: In the puerarin group, the left ventricular summit pressure, maximum rate of intraventricular pressure rise of left ventricular, maximum rate of intraventricular pressure down of left ventricular, and GSHPx/LPO in serum were all increased significantly( P <0 05 or P <0 01), serum LPO decreased significantly( P <0 05), LPO in myocardial tissue decreased, SOD and GSHPx/LPO in myocardial tissue increased significantly (all P <0 05) compared to the model group. Conclusion: Puerarin has the protective effect on ischemia/reperfusion injured myocardium by scavenging oxygen free radicals and antilipid peroxidation reaction.