摘要
目的 :探讨脓毒症时肠道组织肿瘤坏死因子 α( TNFα)、单核细胞趋化蛋白 1( MCP 1)及核因子 κB( NFκB)活性的相关性 ,揭示大黄治疗脓毒症的有效机制。方法 :采用盲肠结扎穿孔术制备大鼠脓毒症模型。分别在术后 1、3、6、12、2 4、4 8h活杀大鼠取肠黏膜组织 ,用酶联免疫吸附法测 TNFα、MCP 1的含量 ,用凝胶电泳迁移法测 NFκB的活性。在脓毒症模型基础上加用大黄治疗 ,分别于治疗后 12、2 4、4 8、72 h活杀大鼠 ,用同样的方法检测 TNFα、MCP 1含量及 NFκB活性。结果 :脓毒症大鼠肠黏膜 NFκB活性及TNFα、MCP 1含量均较相应对照组明显升高 ( P均 <0 .0 1) ;大黄治疗后能明显抑制升高的 NFκB活性和TNFα、MCP 1含量 ( P均 <0 .0 5 )。结论 :TNFα、MCP 1是脓毒症早期激活的细胞因子 ,其释放与NFκB活性密切相关 ;大黄可通过抑制 NFκB活性。
Objective: To investigate the relativity of tumor necrosis factorα (TNFα), monocyte chemotasis protein 1(MCP1) and the activity of nuclear factorκB(NFκB) in the intestinal tissue in sepsis rats, and the mechanism of rhubarb on sepsis. Methods: The sepsis model of rat was established by cecum ligation and puncture. The rats were killed at 1, 3, 6, 12, 24 and 48 hours after operation and the intestinal mucosa tissue was sampled. The contents of TNFα and MCP1 were detected by enzyme linked immunoadsorbent assay, and the activity of NFκB was detected by electrophoresis mobility shift assay. Sepsis rats were treated with rhubarb, then were killed after 12, 24, 48 and 72 hours, the contents of TNFα and MCP1 as well as the activity of NFκB were detected by the same methods as above. Results : Compared to the control group, the activity of NFκB, the contents of TNFα and MCP1 of intestinal mucosa were increased in sepsis rats(all P <0 01). Rhubarb possessed the effect of inhibiting the increase of TNFα , MCP1 levels and NFκB activity(all P <0 05). Conclusion: TNFα and MCP1 are the cytokines activated in the early stage of sepsis, and the release of them is closely relative to the activation of NFκB . Rhubarb ossesses the action of inhibiting inflammatory reaction by inhibiting NFκB activation and reduce the release of inflammatory cytokines.
出处
《中国中西医结合急救杂志》
CAS
2004年第6期364-367,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
辽宁省大连市科技计划项目 (2 0 0 3 B3 NS2 14 )
