食管鳞癌中CD44v4/5、CD44v6的表达与浸润转移的关系

The Relationship Between the Expressions of CD44v4/5, CD44v6 and the Infiltration and Metastasis of Esophageal Squamous Cell Carcinoma

目的 探讨食管鳞癌中CD44v4/5、CD44v6的表达与浸润转移的关系。方法 用免疫组化LSAB法检测了 42例食管鳞癌中CD44v4/5、CD44v6的表达。结果 CD44v4/5在淋巴结转移组的阳性表达率为 76 19% (16/2 1) ,而在非转移组的阳性表达率为42 86% (9/2 1) ,两组之间差异显著 (P <0 0 5 )。CD44v6在淋巴结转移组的阳性表达率为 71 43 % (15 /2 1) ,而在非转移组阳性表达率为 3 8 0 9% (8/2 1) ,两组之间亦具有显著差异 (P <0 0 5 )。从Ⅰ级癌到Ⅲ级癌 ,CD44v4/5的阳性率依次为 69 2 3 % (9/13 )、64 71%(11/17)和 41 67% (5 /12 ) ,彼此间无显著差异 (P >0 0 5 )。CD44v6的阳性率依次为 76 92 % (10 /13 )、5 2 94% (9/17)、3 3 3 3 % (4 /12 ) ,各组之间无显著差异 (P >0 0 5 )。CD44v4/5和CD44v6的阳性率之间差异无显著性 ,也无相关关系。癌巢周围向间质浸润的癌细胞、肌间浸润的癌细胞 ,有核分裂的癌细胞和癌栓中的癌细胞及浸润血管壁的癌细胞CD44v4/5和CD44v6均呈强阳性表达。结论 CD44v4/5和CD44v6的表达均与食管鳞癌浸润转移有关。

Objective To explore the relationship between the expressions of CD44v4/5, CD44v6 and the infiltration and metastasis of esophageal squamous cell carcinoma. Method Using labbelled Streptavidin-Biotin (LSAB) technique, we examined the expressions of CD44v4/5, CD44v6 in 42 cases with esophageal squamous cell carcinomas specimens. Results In the cases with metastasis the positive rate of CD44v4/5 was 76.19%(16/21),but in the cases with non-metastasis the positive rate was 42.86%(9/21), there was a significant difference between the two groups (P<0.05).The positive rate of CD44v6 expression was 71.43%(15/21) in the lymphnode metastasis group, and 38.09%(8/21) in non-metastasis group ,there was a significant difference between the two groups (P<0.05).From grade Ⅰ to grade Ⅲ in differentiation, the positive rates of CD44v4/5 were 69.23% (9/13)、64.71% (11/17) and 41.67%(5/12)respectively, no significant difference presented among them (P>0.05). The positive rates of CD44v6 expression in grade Ⅰ,Ⅱ, Ⅲ squamous cell carcinoma were 76.92%(10/13),52.94%(9/17) and 33.33%(4/12) respectively. There was no significant difference among the three groups (P<0.05). There were no difference and relationship between the rates of CD44v4/5 and CD44v6. Those cancer cells in the margins of the cancer nests, the infiltrated muscules, with mitotic figures, in carcinoma emboli and infiltrating into vascular walls strongly expressed CD44v4/5, CD44v6 positively. Conclusion there was a positive correlation between the expressions of CD44v4/5, CD44v6 and the infiltration and metastasis of esophageal squamous cell carcinoma.