低频超声对Molt-4肿瘤细胞的生物学效应

Bio-effects of Molt-4 cells by low frequency ultrasound

目的 研究低频超声对Molt 4肿瘤细胞的生物学效应 ,评价低频超声在肿瘤治疗中的应用价值。方法 应用流式细胞术和共聚焦显微镜技术研究低频超声 ( 2 0kHz ,0 .2W /cm2 )持续辐照Molt 4肿瘤细胞 1分钟后的细胞凋亡率和细胞凋亡形态学改变 ,以及细胞凋亡增殖比 (APR)的变化。结果 对照和辐照组细胞凋亡率分别为 ( 2 .5 6± 0 .61) %和 ( 11.0 6± 1.99) % (P <0 .0 1)。细胞凋亡具有特征性形态学改变 ,APR上调。结论 低频超声在体外可诱导Molt 4肿瘤细胞凋亡和抑制肿瘤细胞增殖 。

Objective To observe the bio-effects of Molt-4 cells treated with low frequency ultrasound, and to study the feasibility of cancer treatment by low frequency ultrasound. Methods The changes of percentage and morphology of apoptosis and ratio of apoptosis to proliferation (APR) after ultrasonic exposure (20 kHz, 0.2 W/cm^2, 1 min) were analyzed with FCM and confocal imaging. Results The percentage of apoptosis in control group (n=8) and interventional group (n=8) was (2.56±0.61)% and (11.06±1.99)%, respectively (P<0.01). The apoptic cells had characteristic changes in morphology by confocal. The ratio of apoptosis to proliferation was up-regulated. Conclusion Low frequency ultrasound may offer conference of cancer therapy with its capacity of inducing cancer cells' apoptosis and inhibiting its proliferation in vitro. [