去磷酸化RB蛋白在乳腺癌细胞凋亡中的作用

The role of dephosphorylated RB protein in human breast cancer cell apoptosis

目的 :研究去磷酸化RB蛋白表达在人乳腺癌细胞凋亡中的作用。 方法 :以乳腺癌细胞株MCF 7/S(化疗敏感细胞株 )和MCF 7/Adr(耐多柔比星细胞株 )为研究对象 ,采用免疫细胞化学法检测多柔比星 (ADR)作用前、后RB蛋白的表达状态 ;应用流式细胞术检测ADR诱导乳腺癌细胞凋亡程度。 结果 :加ADR前两种细胞胞核中的磷酸化RB蛋白均为阳性 ,去磷酸化RB蛋白均为阴性。经不同浓度ADR处理后 ,随着其浓度的增加 ,MCF 7/S细胞去磷酸化RB蛋白表达量逐渐增高 ,而MCF 7/Adr细胞去磷酸化RB蛋白表达量无明显增高。流式细胞术检测细胞凋亡率和细胞周期 ,结果表明ADR诱导MCF 7/S细胞凋亡作用比MCF 7/Adr强 (P <0 .0 1)。 结论 :去磷酸化RB蛋白表达与MCF 7/S细胞凋亡呈正相关 ,而与MCF

Objective: To investigate the role of the expression of dephosphorylated RB protein for cell apoptosis in human breast cancer. Methods: In our works, human breast cell lines (MCF-7/S,the chemosensitive cell line and MCF-7/Adr, the chemoresistent cell line)were evaluated. The expressive levels of dephosphorylated RB protein were detected with immunocytochemistry. Apoptosis rates were determined by flow cytometry(FCM). Results: Before treatment of ADR, phosphorylated RB protein was positive in two cell lines, but dephosphorylated RB protein was negative; After treatment of different concentration ADR, when the concentration of ADR was increased, expression of dephosphorylated RB protein elevated accordingly in MCF-7/S, but no significant change in MCF-7/Adr. Apoptosis and cycle was detected by FCM assays shows ADR induced apoptosis of MCF-7/S more than MCF-7/Adr(P<0.01). Conclusion: There were positive correlation the expression levels of dephosphorylated RB protein and the apoptosis of MCF-7/S cells for Chemosensitivity, but no significant correlation in MCF-7/Adr.