三氧化二砷免疫毫微球对膀胱癌细胞系的特异性杀伤作用

Specific killing effect of arsenic trioxide-loaded albumin immuno-nanospheres on bladder tumor cells in vitro

目的 验证单克隆抗体BDI 1导向的三氧化二砷免疫蛋白微球 [As2 O3 (HAS NS) BDI 1]对膀胱癌细胞BIU 87的特异性杀伤作用。 方法 通过还原电泳 (SDS PAGE)鉴定免疫蛋白微球抗体BDI 1与微球的共价连接 ,用3 氢 胸腺嘧啶核苷 (3 H TDR)掺入法检测As2 O3 (HAS NS) BDI 1的细胞杀伤活性 ,流式细胞计数法测定细胞凋亡与分期。 结果 SDS PAGE还原电泳可见As2 O3 (HAS NS) BDI 1组有 2条条带。3 H TDR掺入法进一步验证了其特异性杀伤膀胱肿瘤细胞的活性。肿瘤细胞被诱导凋亡 ,G0 /G1期细胞百分比减少 ,G2 /M期百分比明显增加 ,Sub G1期细胞 2 4h增高到 18.4 %。 结论 As2 O3 (HAS NS) BDI 1能特异性地与膀胱肿瘤细胞结合并产生更加有效的杀伤肿瘤细胞的作用。

Objective To study the specific killing effect of arsenic trioxide-loaded albumin immuno-nanospheres [As 2O 3-(HAS-NS)-BDI-1] targeted with monoclonal antibody BDI-1 on bladder tumor cells BIU-87. Methods Covalent bond of As 2O 3-(HAS-NS)-BDI-1 was identified by SDS-PAGE reduction electrophoresis.The 3H-TDR incorporation methods were used to determine the specific killing activity of As 2O 3-(HAS-NS)-BDI-1 in vitro.Cell apoptosis and cycles were observed by FCM. Results The As 2O 3-(HAS-NS)-BDI-1 section yielded two bands on the SDS-PAGE reduction electrophoresis. 3H-TDR incorporation showed the specific killing activity of As 2O 3-(HAS-NS)-BDI-1 on bladder tumor cells.Cell cycle analysis showed that G0/G1 phase cell percentage decreased and G2/M phase cell percentage increased remarkably.Sub-G1 phase cells percentage increased to 18.4% at 24 h. Conclusions As 2O 3-(HAS-NS)-BDI-1 might specifically bind against bladder tumor cell BIU-87 and show high activity of killing bladder tumor cells.