巴戟天对骨髓基质细胞向成骨细胞分化过程Cbfα1表达的影响

Effect of Morinda Officinalis on the Expression of Cbfa1 During the Differentiation from BMSCs to the Osteoblast

目的 :探讨巴戟天对骨髓基质细胞向成骨细胞分化过程 Cbfα1表达的影响。方法 :采用经典成骨诱导方法培养 BMSCs,分别在 3、6、9、1 2、1 5、1 8天通过 RT-PCR方法进行半定量分析 Cbfα1的表达 ,然后分 A组 (巴戟天水提物组 )、B组 (巴戟天醇提物组 )、C组 (对照组 )、D组 (经典成骨诱导 )、E组 (巴戟天水提物 +经典成骨诱导组 )、F组 (巴戟天醇提物 +经典成骨诱导组 )培养 ,在 Cbfα1 m RNA的表达最强的时间检测上述各组 Cbfα1 m RNA的表达。结果 :经典成骨诱导方法诱导至第 3天的时间点开始观察到 Cbfα1 m RNA的表达 ,并逐渐加强 ,至第 1 2天达到高峰 ,1 5天、1 8天有所减弱 ,在 Cbfα1 m RNA的表达最强的时间点 (第 1 2天 ) ,A组、B组、D组、E组、F组均较 C组的表达强 ,均有显著性差异 ,其表达强弱顺序是 :F组 >E组 >D组 >B组 >A组 >C组。结论 :巴戟天水提物、巴戟天醇提物能使 Cbfα1的表达加强 ,且巴戟天醇提物表达强于巴戟天水提物。

Objective:To study the effect of Morinda officinalis How on the expression of Cbfα1 in BMSCs during the differentiation from bone marrow stromal cells to the osteoblast.Methods:According to the classically osteogenic induction method, bone marrow stromal cells of SD rats were separated and cultured, then detect the expression of Cbfα1 after 3,6,9,12,15,18 days by RT-PCR, and observed the time when the expression of Cbfα1 was strongest. On the time when the expression of Cbfα1 was strongest, they were divided randomly into six groups: group A, used aqueous extract of Morinda officinalis How .group B, used alcohol extract of Morinda officinalis How. Group C, used nothing. Group D, used aqueous extract of Morinda officinalis How and classically osteogenic induction method. group E, used alcohol extract of Morinda officinalis How and classically osteogenic induction method group F, classically osteogenic induction method then observed the expression of Cbfα1 in each groups.Results:3 days after classically osteogenic induction, we can observe the expression of Cbfα1 in BMSCs, then become stronger, on 12 days we observed that the expression of Cbfα1 was strongest. On the time when the expression of Cbfα1 was strongest(12 days), the expressions of Cbfα1 in group A、group B、group D、group E、group F were stronger than group C. The order is group F>group E>group D>group B>group A>group C.Conclusions:Aqueous extract of Morinda officinalis How、alcohol extract of Morinda officinalis How can make the expression of Cbfα1 in BMSCs stronger, and alcohol extract of Morinda officinalis How is betterthan the aqueous extract of Morinda officinalis How.