高效液相色谱法测定枇杷叶提取物中齐墩果酸与熊果酸的含量

Determination of oleanolic acid and ursolic acid in Eriobotrya japonica(Thumb.)Lindl extractive by HPLC

目的 :建立同时测定枇杷叶提取物中齐墩果酸与熊果酸含量的方法。方法 :采用HPLC法。选用C18柱 (2 5 0mm×4 .6mm ,5 μm) ;检测波长 :2 2 0nm ;流动相 :甲醇 水 (94∶6 ,每 10 0mL加 0 .0 8mL冰醋酸 ) ;流速 :0 .6mL·min-1;柱温 :2 5℃。结果 :齐墩果酸进样量在 0 .4 5～ 2 .70 μg范围内 ,熊果酸进样量在 0 .4 6～ 2 .76 μg范围内线性关系良好。齐墩果酸与熊果酸的平均回收率分别为 98.3% (RSD为 3.15 % )和 97.7% (RSD为 2 .97% )。结论 :该法方便、快速、准确 。

OBJECTIVE To establish a method for determination of oleanolic acid and ursolic acid in Eriobotrya japonica (Thumb.)Lindl extractive.METHODS HPLC method was used.The samples were separated on a C 18 (250 mm× 4.6 mm , 5 μm )column with the mobile phase of methanol water(94∶6),which contained 0.08% lacial acetic acid.The detection wavelength was set at 220 nm .Flow rate was 0.6 mL·min -1 .Column temperature was 25 ℃ .RESULTS The linear ranges of determination of oleanolic acid and ursolic acid were 0.45 - 2.70 μg and 0.46 - 2.76 μg respectively.The average recoveris of 98.3% with RSD of 3.15% ,for oleanolic acid and 97.7% with RSD of 2.97% for ursolic acid were obtained.CONCLUSION The method is sensitive,rapid and accurate and suitable for the determination of oleanolic acid and ursolic acid in Eriobotrya japonica (Thumb.)Lindl extractive.