反相高效液相色谱法测定复方鼻用软膏中磺胺嘧啶含量

Determination of sulfadiazine in compound nasal ointment by RP-HPLC

目的:建立复方鼻用软膏中磺胺嘧啶的含量测定方法.方法:反相高效液相法,日本岛津Shim-pack VP-ODS柱(250L×4.6),国产C18保护柱,流动相:甲醇-水-冰醋酸(40:60:0.1),检测波长:270nm,流速0.6mL/min.结果:在11.26～123.82μg/mL范围内成线性关系,y=144 647X-41 558,r=0.999 9,平均回收率为100.2±1.5%,RSD为1.4%.结论:本方法简单、准确、快速,可用于本制剂磺胺嘧啶的快速定量分析.

Objective: To establish a method for determination of the content of sulfadiazine in compound nasal ointment. Methods:RP-HPLC was adopted with Shim-pack VP-ODS(250L ×4. 6) column as analytical column, methanol-water-aceti-cacid (40 : 60 : 0. 1) as mobile phase. The detection wavelength was 270nm. The flow rate was 0. 6mL/min. Results :The linear ranges of sulfadiazine were 11. 26 -123. 82μg/mL,Y = 144 647X-41 558,r =0.999 9,the average recovery was (100.2 ±1.5)% ,RSD was 1.4%(n=6). Conclusion: The method is simple, rapid, accurate and suitable for the determination of sulfadiazine in compound nasal ointment.