HPLC法测定灰指灵药膏中间苯二酚、水杨酸、苯甲酸的含量

Determination of resorcinol, salicylic acid, benzoic acid in hueizhilin ointment by high-performance liquid chromatography

目的:建立灰指灵药膏中间苯二酚、水杨酸、苯甲酸的含量测定方法.方法:采用高效液相色谱法,以C18为固定相,甲醇-磷酸二氢钠(40:60)为流动相,测定波长为254nm.结果:间苯二酚、水杨酸、苯甲酸分别在40.0～200.0μg/mL范围内,其峰面积值与浓度具有良好的线性关系(r=0.999 9、0.999 7、0.999 9),平均回收率分别为98.1%、97.9%、97.7%.RSD分别为1.88%、1.82%、1.46%.结论:该方法可靠,精密度高,可作为该制剂的质量控制方法.

Objective: To establish a method of determination of contents in Hueizhilin ointment. Methods: HPLC was used with C18 column as stationary phase and methanol-sodium dihydrogen phosphate (40 : 60) as mobile phase. The detection wavelength was 254nm. Results:The calibration curve is linear between 40. 0 -200. 0μg/mL of resorcinol salicylic acid and benzoic acid . The average recorery was 98. 1% , 97. 9% ,97. 7% with RSD of 1. 88% ,1. 82% ,1. 46% . Conclusion:The method is reliable, accurate and can be used for the determination of the contents of Hueizhilin ointment .