Pre-S2蛋白抗原表位及二级结构的预测与比较

Prediction and Comparison of Secondary Structures and Epitopes for Pre-S2 Region of Different Subtype HBV

本文采用Chou和Fasman方法预测adw,adr,ayw三种亚型的乙肝病毒Pre-S2蛋白的二级结构,并用双亲性方案和亲水性方案分析了抗原表位。结果显示三者均可能含有较多的β-片层和β-转折;N-末端30个残基所形成的二级结构较保守,而C-末端顺序的构象在亚型间差别较大;Th细胞识别的表位可能在39—49肽段,B细胞识别的表位可能主要在13—18残基或其附近,为分子免疫学的深入研究提供了线索。

The secondary structures of pre-S2 region of adw, adr, ayw subtype HBV were predicted by Chou and Fasman's method, and the antigenic epitopes were analysed by using amphipathic plot and hy-drophilicity plot. The results showed that all the three subtypes are rich in f}-sheets and |3-turnsithe conformation of the N-terminal sequence is highly conserved. but of the C-terminal sequence is of polymorphism i the epitope recognized by Th cells may be at 39 - 49, and by B cells at or adjacent to 13 - 18.