正常人肝细胞HBV PreS2相关抗原的研究

Study of HBV-PreS2 Antigen Associated on the Normal Hepatocytes

经斑点免疫印迹试验和Western Blot,用抗P31和抗PreS120-145研究了HBV包膜蛋白抗原同正常人肝细胞膜抗原的关系。结果表明:肝膜纯化物能与抗P31及抗PreS120-145反应,其中抗PreS120-145只能识别肝膜抗原中的两条蛋白带(123KD,68KD)。提示在正常人肝细胞膜上存在有HBV-PHSAr的相关抗原,这种抗原在参预HBV嗜肝过程和乙型肝炎的免疫病理损伤中可能起重要作用。

The antigenic relationships between HBV envelop protein and normal human hepatocytes, preparations were investigated by using antibodies specific for P31 (natural PHSA binding protein) and PreS120 -145(synthetic PHSA binding site)epitopes. The hepatocyte and membrane antigen preparations ,o-riginating from liver of donators whose serum have no HBV infection markers,were isolated and further purified by gradient as well as sucrose density gradient centrifugations. In Dot-immuno-binding-assay, we found that antigen preparations highly reacted with anti-P31 and anti-PreS120-145. The results also showed that the expression of these cross-reaction antigens in individuals is variable .because the densities of spots in DIA were different even though protein quantity on each dot was the same. The preparations after separation by SDS-PAGEC7. 5%) and staining with Coomassie Blue,anti-PreSl20 -145 reacted with only two bands,one's molecular weight is about 123 000 Da,the other 68 000 Da. Our researches suggested that host cells might play role in the process of HBV hepatotrophic infection and immunological liver injuries of hepatitis B.