手十二井穴刺络放血法对MCAO模型大鼠大脑皮层c-fos蛋白表达的影响

The Influence of Blood-letting Puncture of Twelve Jing-Well Points on Corticocerebral C-fos Protein Expression in Rat MCAO Model

目的 从脑内 c- fos蛋白表达的角度观察井穴放血法对急性缺血性脑损伤的脑保护作用。方法 以凝结法阻断大鼠一侧大脑中动脉造成急性局灶性缺血性脑损伤模型 (MCAO) ,用免疫组织化学法观察缺血区皮层脑组织 c- fos阳性细胞的表达变化及施加手十二井穴放血法后对其的干预治疗作用。结果 急性缺血性脑损伤可快速诱导 c- fos蛋白在皮层缺血区脑组织的表达 ,其表达有随缺血时间延长逐渐增多的趋势 ,而缺血后即刻施加井穴放血的干预治疗 ,在 1h、3h、6 h、2 4 h各缺血时间段均可明显上调缺血区皮层脑组织 c- fos蛋白的表达水平。结论 c- fos蛋白表达既与缺血的严重程度有关 ,也反应神经元的应激反应能力 ,脑缺血能诱导 c- fos蛋白的逐渐表达 ,提示脑的缺血性损伤可应激性地增强神经细胞对缺血、缺氧的耐受和适应能力。而早期施加井穴刺络放血疗法能进一步增强 c- fos蛋白表达 ,提高神经细胞的应激能力 ,从而提高缺血区脑组织的抗损伤修复能力 ,减缓神经细胞凋亡 ,抵抗缺血性脑损害的进一步发展 ,产生有效的脑保护作用 ,表明井穴放血法对中风早期有一定的急救作用。

Objective To investigate the preventive effect of blood-letting puncture of twelve Jing-Well points on acute ischemic cerebral lesion from intracerebral c-fos protein expression. Methods A rat model of acute ischemic cerebral lesion was made by coagulation occlusion of unilateral middle cerebral artery. Cerebral c-fos positive cell expression in the ischemic cortex was observed by immunohistochemical method and the preventive effect of blood-letting puncture of twelve Jing-Well points on the lesion was investigated. Results Acute ischemic cerebral lesion could quickly induce cerebral c-fos protein expression in the ischemic cortex, which tended to increase gradually with the prolongation of ischemic time. Blood-letting puncture of Jing-Well points as an intervention immediately after ischemia could significantly up-regulate cerebral level of c-fos protein expression in the ischemic cortex at 1,3,6 and 24 hrs after the ischemia. Conclusion C-fos protein expression is not only related to the severity of ischemia but also reflect the responsive ability of neurons. Cerebral ischemia can induce gradual expression of c-fos protein, suggesting that cerebral ischemic lesion can improve the ability of neurons to tolerate and adapt to ischemia and anoxia by a stress reaction and that early blood-letting puncture of Jing-Well points can further increase c-fos protein expression and improve the stress ability of neurons to ameliorate the ability of ischemic brain tissues to prevent the lesion and make repairs, slow neuronal apoptosis, prevent the further development of ischemic cerebral lesion and produce an effective protecting action on the brain. It is showed that blood-letting puncture of Jing-Well points has a certain first-aid effect on the early stage of stroke.