β-淀粉样蛋白25-35片段诱导PC12细胞凋亡

Apoptosis of PC12 cell Induced by Amyloid Beta-peptide Fragment 25-35

目的 探讨 β -淀粉样蛋白 2 5 - 35片段 (Aβ2 5-3 5)对体外培养的PC12细胞的毒性作用机制。方法 用四甲基偶氮唑蓝 (MTT)代谢率检测 ,光镜吖啶橙荧光染色术 ,透射电镜以及流式细胞仪技术研究Aβ2 5-3 5损伤PC12细胞的途径。结果 用Aβ2 5-3 5处理PC12细胞 2 4h ,Aβ2 5-3 5剂量依赖性地引起PC12细胞的MTT代谢率减少 ,荧光染色及电镜观察发现经Aβ2 5-3 5处理的PC12细胞表现出凋亡细胞的特征 ,流式细胞仪检测发现 ,对照组 2 0 μmol/L及 5 0 μmol/L的Aβ2 5-3 5组PC12细胞的凋亡率分别为 0 .0 8%± 0 .0 1% ,14 .8%± 1.13% ,2 5 .9%± 2 .34%。

Purpose: To study the neurotoxicity of amyloid beta-peptide fragment 25 - 35 (AfJ25 _35) in cultured PC12 cells. Methods: The way of Aβ25- 35 induced PC12 cells injury was analyzed by MTT metabolic rate examination, acridine orange fluorescence staining light microcopy, transmission electron microscopy and flow cytometry. Results: Treatment of PC12 cells with Aβ25-35 for 24 h caused a dose-dependent decrease in MTT metabolic rate examination, morphological apoptosis of PC12 cells could been seen under fluorescence microscope and electron microscope, respectively, proportions of apoptotic cells of control group, 20 μmol/L and 50 μmol/L Aβ25-35 group were 0.08% ± 0.01%, 14.8% ± 1.13%, 25.9% ± 2.34% by flow cytometry. Conclussions: Aβ25-35 injured PC12 cells mainly via apoptotic pathway.