轻度认知损害病人脑脊液白细胞介素-1和肿瘤坏死因子水平的研究

Detection of Interleulin-1β and Tumor Necrosis Factor a in Cerebrospinal Fluid in patients with Mild Cognitive Impairment

目的:为探讨炎性细胞因子在轻度认知损害(mild cognitive impairment,MCI)慢性炎性病理过程中的作用。方法:本研究采用双抗体夹心酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)分别对AD对32例MCI病人与15例正常对照的脑脊液(cerebrospinal fluid,CSF)的自细胞介素IL-1β(Interleukin-1β,IL-1β)和肿瘤坏死因子α(tumor necrosis factors alpha,TNFα)的水平进行了测定。结果:MCI组与正常对照相比,MCI组病人CSF中IL-1β水平和TNFα水平明显升高。结论:上述结果表明,炎性细胞因子IL-1β和TNFα在MCI的神经变性过程起着重要的作用,炎症参与MCI向AD转化发病机制。观察MCI患者CSF的IL-1β水平和TNFα水平可以为早期诊断和治疗提供依据。

Objective: To analyse the cerebrospinal fluid values of the interleulin-1β and tumor necrosis factor a in patients with mild cognitive impairment. Method: The levels of the interleulin-1β and tumor necrosis factor a were analyzed using ELISA in 32patients with MCI and in 15 age matched healthy controls. Result: Patients with MCI displayed significantly higher levels of interleulin-1β and tumor necrosis factor a compared with the healthy controls. Conclusion: These results suggest that premflammatory cytokines interieulin-1β and tumor necrosis factor a should be important factors in the neurodegeneration progress and the inflammation should significantly contribute to trandform MCI into AD. The detection of the level of the interleulin-1β and tumor necrosis factor a in CSF should be valuable to MCI early diagnosis and treatments.