溶血卵磷脂对胰腺炎大鼠脑损害作用的实验研究

A research on the brain damage of lysophosphatidyl choline in rats with pancreatitis

目的 :从溶血卵磷脂 (LPC)开放胰腺炎大鼠血脑屏障 (BBB)以及造成其脑部脱髓鞘两方面探讨LPC对胰腺炎大鼠的脑损害作用。进一步揭示胰性脑病 (PE)的发病机制。方法 :SD大鼠 30只随机分 3组 ,用Aho法将实验组、对照组 1制成急性水肿型胰腺炎模型 ,随后对对实验组大鼠尾静脉应用LPC 5d ,对照组一从尾静脉注入生理盐水。对照组 2是非手术组 ,对其进行尾静脉注入LPC。LPC注射结束后 7～ 10d ,应用辣根过氧化物酶———二氨基联苯胺显色法 (HRP DAB法 )检测其BBB是否开放 ,同时行脑组织切片丽春红、固兰染色观察有无脱髓鞘改变。结果 :实验组大鼠的BBB开放和脱髓鞘均较明显 ,对照组的BBB开放、脱髓鞘均不明显 ,均有具有统计学差异。结论 :LPC能开放胰腺炎大鼠的BBB且能造成脱髓鞘损害 。

Objective: To explore the brain damage effects of lysophasphatidyl choline(LPC) in rats with pancreatitis and explore the pathogenesis of pancreatic encephalopathy(PE).Methods: SD rats were divided into test group,control group one and control group two at random.Acute edematous pancreatits rat model was induced following Aho HJ method for test group and control group one,then test group rats were venously applied with LPC and control group one was injected saline through tail vein.Control group two was treated with venous injection LPC through the rat tails without operation.Horseradish peroxidase(HRP) was used as a tracer to detemine if the blood-brain barrier(BBB)was open 7～10 days after successive application of LPC.The extravasated tracer was showed by diaminobenzidine(DAB).Rat brain tissue sections were examined by ponceau stain and Luxol Fast Blue stain to determine whether the rat brains were demyelination at the same time.Results: The BBB permeability of the test groupLTU rats increased greatly and obvious demyelination was observed in test group rat brains while both control groups had basically intact BBB and scarce demyelination was observed in both control groups.A statistical difference existed between the test group and control groups.Conclusion:LPC definitely open the BBB of the rat pancreatitis and demyelinates the brain of pancreatitis rats thus LPC plays an important role in the pathogenesis of PE.