急性呼吸道疾病爆发流行的病原学研究

An etiology study on the outbreak of acute respiratory disease (ARD)

目的 对 2 0 0 4年 4月起在江苏苏北及其邻近地区发生中、小学生爆发急性呼吸道疾病(ARD)流行的集中发热并伴有头痛、头晕、咽痛、扁桃体炎病例进行病因研究。方法 对流行区 2 0位患者的咽拭子标本分离病原体 ,并对阳性分离株进行免疫荧光、聚合酶链反应 (PCR)以及电镜检测。结果 MDCK(35代 )、LLC MK2、VeroE6、MRC 5 (39代 )分离培养均未出现细胞病变 ;军团菌培养亦未发现阳性结果 ;经人喉癌传代细胞 (Hep 2 )分离培养 ,2 0份标本中 13份出现了明显的细胞病变效应 ,电镜下可见细胞胞核内大量呈晶格状排列的典型腺病毒颗粒 ,直接免疫荧光试验及PCR检测均证实为腺病毒感染。结论 2 0 0 4年 4月发生在江苏省苏北地区中、小学急性呼吸道感染疫情的病原体为腺病毒。

Objective Etiology study were conducted on 20 cases with acute respiratory disease (ARD). Methods 20 throat swab samples were collected and the virus isolation was engaged with MDCK, LLC-MK2, Vero, MRC-5 and Hep-2 cells. Bacterial isolation work was conducted at the same time, including legionella and blood plate culture. Results 13 ADV-like cytopathic effect were detected in Hep-2 cultures, and negative staining electron microscopy result typical ADV partials. IFA, PCR also gave out positive result. Conclusions Pathogen of this ARD outbreak in the north of Jiangsu province was adenovirus.